响应面法优化超声波辅助提取废弃烟叶中芦丁的研究  

Study on Optimization of Ultrasound-assisted Extraction Technology of Rutin from Waste Tobacco Leaves by Response Surface Methodology

作　　者：李海洋[1] 李莹[1] 李荣华[1] 吕永华[2] 夏岩石[1] 李钟[3] 郭培国[1] 

机构地区：[1]广州大学生命科学学院,广东广州510006 [2]广东省烟草专卖局,广东广州510610 [3]广东药科大学中药学院,广东广州510006

出　　处：《食品研究与开发》2017年第5期80-84,共5页Food Research and Development

基　　金：广东省烟草专卖局(公司)科技计划项目(201310)

摘　　要：以烟草废弃烟叶作材料,根据Box-Behnken中心组合试验设计原理,选取提取温度、超声时间、乙醇体积分数作为优化因素,在单因素试验的基础上,采用响应面法对废弃烟叶中芦丁的提取工艺进行优化。试验结果表明芦丁提取的最佳工艺参数为:提取温度57.5℃、超声时间20.3 min和乙醇体积分数32.6%,提取率为13.7 mg/g,与理论预测值的相对误差为1.0%,说明响应面法可以很好地优化废弃烟叶中芦丁的提取工艺。这一结果可为烟草废弃物的综合利用提供参考。Based on single factor experiments for extraction temperature, ultrasonic time and volume ratio of ethanol, the optimum extraction techniques of rutin from waste leaves was performed through the analysis of re- sponse surface method （RSM） which was designed by Box-Behnken Design （BBD）. The experimental results showed that the optimum extraction parameters were as follows： extraction temperature at 57.5 ~C, ultrasonic time for 20.3 min and 32.6 % of ethanol volume ratio. The average extraction yield of rutin was 13.7 mg/g with 1.0 % of the relative error comparing with the theoretical value, demonstrating that response surface method could effectively optimize the extraction process of rutin from waste tobacco leaves. This result could provide ref- erences for the comprehensive utilization of tobacco waste.

关 键 词：响应面法 废弃烟叶 芦丁 提取 

分 类 号：R284.2[医药卫生—中药学]