血清SOCS-3、TNF-α、IL-16水平及Th1/Th2与妊娠期高血压疾病的关系  

Relationship between serum SOCS-3,TNF-α,IL-16,Th1/Th2 ratio and hypertensive disorder complicating pregnancy

作　　者：温丽[1] 郭珍[1] 胡际东[1] 田新羽 王丽丽[1] 常春红[1] 周欢欢[1] 

机构地区：[1]河北涿州市医院妇产科,河北涿州072750

出　　处：《解放军医学院学报》2017年第5期426-429,432,共5页Academic Journal of Chinese PLA Medical School

基　　金：保定市科学计划与发展指导计划项目(16ZF052)~~

摘　　要：目的探讨血清细胞因子信号转导负调控因子-3(suppressor of cytokine signaling 3,SOCS-3)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-16(interleukin-16,IL-16)及T辅助细胞1/T辅助细胞2(Th1/Th2)与妊娠期高血压疾病(HDCP)的关系。方法选取2014年1月-2016年6月本院妇产科收治的妊娠期高血压疾病患者182例,其中妊娠期高血压组51例,轻度子痫前期组69例,重度子痫前期组62例。另选择同期分娩健康孕妇65例作为对照组。采用ELISA法检测各组孕妇血清SOCS-3、TNF-α、IL-16及Th1/Th2水平,分析重度子痫前期患者不良妊娠结局与各指标的关系。采用Spearman相关分析各组HDCP患者血清SOCS-3水平与TNF-α、IL-16及Th1/Th2的相关性。结果与对照组比较,轻度子痫前期组和重度子痫前期组血清SOCS-3水平均明显降低(0.59±0.12 vs 0.14±0.03和0.08±0.01;P均<0.05)。各组TNF-α、IL-16及Th1/Th2水平均高于对照组,且重度子痫前期组较轻度子痫前期组升高更明显[TNF-α(ng/L):12.58±2.37 vs 34.75±6.42和61.53±9.26;IL-16(ng/L):108.47±35.24 vs 187.63±81.47和284.62±113.58;Th1/Th2:9.27±2.38 vs20.15±4.82和26.48±6.13;P均<0.05]。重度子痫前期患者中,SOCS-3低表达组胎儿宫内窘迫综合征、胎儿生长受限及新生儿窒息的发生率均显著高于高表达组(P均<0.05),TNF-α、IL-16、Th1/Th2高表达组早产及胎儿生长受限的发生率均显著高于低表达组(P均<0.05)。相关分析显示,妊娠期高血压患者血清SOCS-3水平与TNF-α、IL-16、Th1/Th2均呈负相关(r=-0.528,r=-0.506,r=-0.126;P均<0.05)。结论监测孕妇血清SOCS-3、TNF-α、IL-16及Th1/Th2对妊娠期高血压疾病尤其是重度子痫前期的早发现、早诊断、早治疗具有一定的临床指导意义。Objective To investigate the relationship of serum cytokine signaling negative regulation factor-3（SOCS-3）,tumor necrosis factor-α（TNF-α）,interleukin-16（IL-16） and T helper cell 1/T helper 2（Th1/Th2） ratio with hypertensive disorder complicating pregnancy（HDCP）.Methods From January 2014 to June 2016,182 cases with hypertensive disorder complicating pregnancy were selected from the department of obstetrics and gynecology in our hospital,including 51 cases of hypertensive of pregnancy,69 cases of mild preeclampsia and 62 cases of severe preeclampsia.Another 65 cases of healthy pregnant women were selected as the controls.The levels of serum SOCS-3,TNF-α,IL-16 and Th1/Th2 in each group were detected by ELISA assay,and the relationship between the adverse pregnancy outcome and the serum indexes in patients with severe preeclampsia were analyzed.The correlation of serum SOCS-3 levels with TNF-α,IL-16 and Th1/Th2 were examined by spearman correlation analysis.Results Compared with the control group,the serum levels of SOCS-3 were significantly lower in the mild preeclampsia group and the severe preeclampsia group（0.59±0.12 ng/L vs 0.14±0.03 ng/L and 0.08±0.01 ng/L,P〈0.05）.The levels of TNF-α,IL-16 and Th1/Th2 were 19.13±4.36,143.26±57.82,16.58±3.64 in gestational hypertension group,34.75±6.42,187.63±81.47,20.15±4.82 in mild preeclampsia group,and 61.53±9.26,284.62±113.58,26.48±6.13 in severe preeclampsia group,which increased by disease severity（P〈0.05,respectively）,and were significantly higher than those in control group（12.58±2.37,108.47±35.24,9.27±2.38）（P〈0.05,respectively）.The incidences of fetal distress syndrome,fetal growth restriction and neonatal asphyxia in SOCS-3 low expression group were significantly higher than those in the high expression group（P〈0.05,respectively）.The incidences of premature birth and fetal growth restriction in TNF-α,IL-16 and Th1/Th2 high expression groups were significantly higher than those in the low expression g

关 键 词：妊娠期高血压疾病 信号转导负调控因子-3 肿瘤坏死因子-α 白细胞介素-16 T辅助细胞1/T辅助细胞2比值 

分 类 号：R714.252[医药卫生—妇产科学]