血清miR-4762-5p在鉴别Ⅳ型和Ⅳ+Ⅴ型狼疮性肾炎中的临床意义  

Clinical Significance of Serum miR-4762-5p in Differentiating between Class Ⅳ and Class Ⅳ+Ⅴ Lupus Nephritis

作　　者：苏美玲[1] 肖红波[1] 葛雅婷 王艳[1] 黄莉敏[1] 熊祖应[1] 

机构地区：[1]北京大学深圳医院肾内科,深圳518036

出　　处：《华中科技大学学报（医学版）》2017年第2期205-209,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：广东省自然科学基金博士启动项目(No.S2013040015040)

摘　　要：目的探讨血清miRNA是否能够作为鉴别Ⅳ型和Ⅳ+Ⅴ型狼疮性肾炎(lupus nephritis,LN)病理类型的指标及其临床意义。方法选取60例血清标本,其中Ⅳ型LN组、Ⅳ+Ⅴ型LN组、对照组各20例。利用既往miRNA array芯片结果筛选出7个在Ⅳ型和Ⅳ+Ⅴ型LN组间有差异表达的血清miRNA,采用qRT-PCR技术初步验证芯片结果,并分析血清miRNA与LN病理类型的关系及其与临床参数的相关性。结果血清miR-4762-5p和miR-151a-3p在不同组间表达量存在差异性。与Ⅳ型LN组、对照组比较,Ⅳ+Ⅴ型LN组血清miR-4762-5p表达量升高(均P<0.05);与对照组比较,Ⅳ型LN组和Ⅳ+Ⅴ型LN组血清miR-151a-3p表达量均下降(均P<0.05)。Ⅳ型LN组血清miR-4762-5p表达量与24h尿蛋白量、肾小球滤过率呈负相关。结论血清miR-4762-5p将有可能作为鉴别Ⅳ和Ⅳ+Ⅴ型LN病理类型的生物学标记。Objective To explore the clinical value of serum miRNA in differentiating between class Ⅳ and class Ⅳ ＋ Ⅴlu- pus nephritis（LN）. Methods The serum samples were harvested from 20 class IV LN patients,20 class Ⅳ ＋ Ⅴ LN patients and 20 healthy individuals. According to the previous miRNA array results obtained by our team, 7 serum miRNAs（miR-664a- 5p,miR-4762-5p, miR-151a-3p, miR-146a-5p, miR-21-5p, miR-130b-5p, miR-3165）which were significantly different between class Ⅳ and Ⅳ ＋Ⅴ LN were screened out. By real-time quantitative PCR,the results of 7 miRNAs were verified. The relationship between serum miRNAs and LN pathological types or clinical indicators was analyzed. Results There were significant differences in serum miR-4762-5p and miR-151a-3p between different groups, miR-4762-Sp was significantly up-regulated in class Ⅳ ＋ Ⅴ LN group compared with the class Ⅳ LN group and the control group（P〈0.05）, miR-151a-3p was significantly down-regulated in both the class IV LN group and class Ⅳ ＋ Ⅴ LN group compared with the control group（P〈0.05）. The ex- pression of miR-4762-5p was negatively related to the 24-b proteinuria and glomerular filtration rate in class IV LN group. Conclusion Serum miR-4762-5p may become one of the biomarkers that can differentiate between class Ⅳ and class Ⅳ ＋Ⅴ LN.

关 键 词：狼疮性肾炎 微小RNA 病理类型 血清 生物学标记 

分 类 号：R593.242[医药卫生—内科学]