乙肝病毒血清标志物与HBV DNA载量及肝功能指标的关联性  

Correlations of Serum Markers of Hepatitis B Virus,HBV DNA Load and Liver Function

作　　者：王斌[1] 黄汉菊[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院检验科,武汉430030 [2]华中科技大学同济医学院基础医学院病原生物学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2017年第2期214-219,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨外周血中乙肝病毒血清标志物(hepatitis B virus serum markers,HBV-M)、HBV DNA载量和反映肝损伤的肝功能指标之间的关联性。方法回顾分析2014年3月~2016年3月同济医院483例慢性乙型肝炎患者资料,HBV-M采用微粒子化学发光技术定量检测;HBV DNA采用实时荧光定量PCR检测;谷丙转氨酶(ALT)和谷草转氨酶(AST)采用紫外连续监测法检测。结果 HBsAg的含量与HBV DNA载量、ALT异常比例(>41U/L)和AST异常比例(>35U/L)之间均无关联性(均P>0.05);HBeAg的含量与HBV DNA载量和ALT异常比例之间均无关联性(均P>0.05),但与AST异常比例有关联,关联程度不密切(r=0.21,P<0.01);抗-HBe的含量与HBV DNA载量和AST异常比例之间有关联性,但关联程度不密切(r=0.16,P<0.05;r=0.19,P<0.01),与ALT异常比例之间无关联性(P>0.05);抗-HBc的含量与HBV DNA载量、ALT异常比例和AST异常比例之间均有关联性,但关联程度不密切(r=0.25,P<0.01;r=0.29,P<0.01;r=0.29,P<0.01);HBV DNA载量的对数值与ALT和AST呈正相关,但线性相关程度较弱(r=0.24;r=0.29)。结论乙肝病毒血清标志物定量检测和HBV DNA定量检测,二者相互补充但不能取代,同时联合肝功能各项指标的检测,更有助于了解肝组织的受损程度。Objective To investigate the correlations of serum markers of hepatitis B virus, HBV DNA load and liver func tion indexeslalanine aminotransferase（ALT）and aspartate transaminase（AST）]in the peripheral blood. Methods Clinical data of 483 patients who were diagnosed with chronic hepatitis B and treated between March 2014 and March 2016 in Tongji hospital were retrospectively analyzed. The serum markers of hepatitis B virus were quantitatively detected by chemiluminescent microp-; article immunoassay. Serum HBV DNA load was measured by real-time fluorescence quantitative PCR, and ALT and AST by continuous ultraviolet monitoring. Results There was no correlation between the HBsAg content and HBV DNA load or the rates of abnormal ALT（〉41 U/L）and abnormal AST（〉35 U/L）（P〉0.05）. The HBeAg content was not correlated with HBV DNA load and the rates of abnormal ALT（P〉0.05） ,but weakly with the rate of abnormal AST（r=0.21 ,P〈0.01）. Although the anti-HBe content was not correlated with the rate of abnormal ALT（P〉0.05） ,it was weakly related to HBV DNA load and the rates of abnormal AST（r = 0.16, P〈0.05 ; r = 0.19, P〈 0.01）. The anti-HBc content had weak correlations with HBV DNA load, the rates of abnormal ALT and abnormal AST（r= 0.25, P%0.01 ;r=0.29 ,P〈0. 01;r= 0.29, P〈0.01）. The logarithm value of HBV DNA load was weakly positively correlated with ALT and AST（r= 0.24; r= 0.29）. Conclusion Quantitative detection of both serum markers and the DNA of hepatitis B virus can complement each other, and when combined with detection of liver function indexes,it will help understand the damage of liver tissue.

关 键 词：乙型肝炎病毒 乙肝病毒血清标志物 HBV DNA 谷丙转氨酶 谷草转氨酶 

分 类 号：R446.62[医药卫生—诊断学]