反式脂肪酸对小鼠大脑抗氧化系统及ATP酶的影响  

Influence of trans-fatty acids on antioxidant system and ATP enzyme in brain of mice

作　　者：孔正桥 刘萍[1] 胡金宇[1] 张甜甜[1] 孙瑜[1] 

机构地区：[1]山东大学公共卫生学院卫生检验学系,山东济南250012

出　　处：《环境与健康杂志》2017年第1期25-27,共3页Journal of Environment and Health

摘　　要：目的探讨反式脂肪酸对小鼠大脑抗氧化系统及ATP酶的影响。方法将48只健康SPF级昆明系雄性小鼠随机分为4组,分别为对照组和25、50、100 mg/kg反式脂肪酸染毒组,每组12只。采用灌胃方式进行染毒,染毒容量均为10ml/kg,每天1次,连续染毒12周。测定小鼠脑组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、ATP酶的活力和丙二醛(MDA)、总蛋白的含量。结果与对照组相比,各剂量反式脂肪酸组小鼠脑组织中的抗氧化酶SOD、GSH-Px、CAT活力均降低,而脂质过氧化物MDA的含量升高;Na^+K^+-ATP酶和Ca^(2+)Mg^(2+)-ATP酶活力均降低,差异均有统计学意义(P<0.05)。结论反式脂肪酸的摄入会引起小鼠脑组织抗氧化系统受损,同时,会降低脑组织中ATP酶的活力。Objective To explore the effect of trans-fatty acids （TFA） on the redox system and ATP enzyme in brain of mice. Methods Forty-eight healthy male Kunming mice of SPF gradewere randomly divided into four groups, 12 in each, the control group, 25 mg/kg, 50 mg/kg and 100 mg/kg trans-fatty acids exposed group. The tested chemicals were given by gavage with exposed capacity of 10 ml/kg, once a day for 12 consecutive weeks. The superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, eatalase （CAT）, ATP enzyme activities and malondialdehyde （MDA）, total protein content in the brain were determined. Results Compared with the control group, trans-fatty acids intake caused significant increase of MDA but reduction of the activities of SOD , GSH-Px and CAT（P〈0.05）. Trans-fatty acids exposure caused a significant reduction of the activities of Na＋K＋-ATPase, Ca2＋Mg2＋-ATPase （P〈0.05）. Conclusion Trans-fatty acid exposure can cause the impairment of the redox system and the reduction of ATP enzyme activity in the brain of mice.

关 键 词：反式脂肪酸 丙二醛 抗氧化酶 ATP酶 

分 类 号：R994.6[医药卫生—毒理学]