免疫印迹试验在丙肝确认检测中的应用  

The Application of Western Blot for Hepatitis C Diagnostic Test

作　　者：袁宗智 陈燕梅[1] 周敏[1] 

机构地区：[1]自贡市疾病预防控制中心,四川自贡643000

出　　处：《预防医学情报杂志》2017年第6期600-602,共3页Journal of Preventive Medicine Information

摘　　要：目的探讨免疫印迹试验在丙肝确认检测中的应用。方法将135份HCV初筛为阳性的血清标本再进行抗-HCV的ELISA检测,仍为阳性的再用荧光PCR法检测HCV-RNA以及特异性更高的补充确认试验,即免疫印迹试验(WB)。结果初检为阳性的135份血清ELISA检测后仍然为阳性,HCV-RNA的检测结果为<1.0×10~3IU/ml有28份,>1.0×10~3IU/ml的有107份。WB结果为阳性115份,阴性19份,不确定1份。结论多种原因可能会导致ELISA法检测丙肝抗体时出现假阳性,而核酸检测灵敏度、病毒血症消退或病毒基因变异等多种因素的影响可能也会出现抗-HCV结果阳性而RNA检测结果为阴性的情况。因此建议对于抗-HCV初检阳性的标本进一步用免疫印迹试验进行复检,以排除假阳性结果。ObjectiveTo explore the application of Western Blot （WB） for hepatitis C diagnostic test. MethodsThere were 135 HCV - antibody screening positive serum samples tested by Laboratory ELISA from clinic cases and the positive samples retested by RT- PCR and WB. ResultsAll HCV -antibody screening positive serum samples were positive by ELISA, but 28 cases were less than 1.0 × 10^3IU/ml with RT - PCR test and 107 cases were greater than 1.0 × 10^3IU/ml. 115 cases were positive by WB test, 19 cases were negative and 1 case unknown. ConclusionThere might be several causes lead the false - positive results of HCV antibodies by ELISA test method. And the anti -HCV was positive but RNA test was negative, which could be affected by nucleic acid detection sensitivity, toxemia and genetic mutation. It is recommended that immunoblotting test should be re -check to exclude the false -positive results.

关 键 词：丙型肝炎 免疫印迹试验 ELISA HCV—RNA 假阳性 

分 类 号：R512.63[医药卫生—内科学]