漆酶催化改性碱木质素与对甲苯酚共聚的研究  

Laccase catalyzing copolymerization of modified alkali lignin and p-cresol

作　　者：王璐瑶[1] 余昭琴 贾红华[1] 周华[1] 韦萍[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800

出　　处：《现代化工》2017年第5期80-84,共5页Modern Chemical Industry

基　　金：国家自然科学基金(21406114)

摘　　要：利用酚化方法对木质素进行修饰,使其接入活性基团,考察了不同的酚单体对木质素改性效果的影响,优化了漆酶催化改性碱木质素和对甲苯酚的共聚反应过程,并对共聚产物进行了表征和性能测试。研究表明,邻苯二酚改性后的碱木质素的酚羟基质量摩尔浓度增加最显著,由6.95 mmol/g提高到10.83 mmol/g,提高了55.83%。共聚反应的最佳反应条件为:反应体系的最适质量分数为80%丙酮溶液,反应温度为30℃,pH 3.5,最适碱木质素与对甲酚质量比为0.5/1,最佳酶浓度为300 U/m L,反应时间为16 h。在最适条件下,共聚物产率显著提高,可达93.6%。邻苯二酚改性后的碱木质素和对甲酚聚合后的产物分子质量明显增加,由2 770 g/mol提高到20 481 g/mol,增加了约10倍,热稳定分析表明聚合产物的热学稳定性比改性前有所提高。Lignin is modified with the phenolation method and inserted with active groups. The influences of various phenolic monomers on the modification of lignin are studied. Then the laccase catalyzing copolymerization reaction between lignin and phenol has been optimized,and the characterization and performance determination on the copolymerization products have been done. The results show that the phenolic hydroxyl content in alkali lignin modified by catechol increases significantly from 6. 95 mmol/g to 10. 83 mmol/g,an increase of about 55. 83%. Moreover,the optimal copolymerization reaction conditions include 80% acetone solution,temperature 30℃,pH 3. 5,substrate ratio of0. 5/1 between alkali lignin and p-cresol,enzyme concentration of 300 U/m L and reaction time 16 h. Under these optimum conditions,the yield of copolymer increases significantly,up to 93. 6%. If catechol modifying lignin is used to copolymerize with p-cresol,the molecular weight of copolymers increases significantly from 2 770 g/mol to 20 481 g/mol,an increase of about 10 times. Thermal stability analysis shows that the thermal stability of copolymer has been improved by the modification.

关 键 词：木质素 酚化改性 漆酶 聚酚树脂 共聚 

分 类 号：TQ031.2[化学工程]