肥胖大鼠心外膜脂肪巨噬细胞数量和血清脂肪因子的研究  

Study on Macrophage in Epicardial Adipose Tissue and Serum Fatty Cytokine Level in Obese Rats

作　　者：张彩坤[1] 张豪英[1] 邢冬杰[1] ZHAGN Caikun ZHANG Haoying XING Dongjie(Department of Western Medicine Education, Shandong College of Traditional Chinese Medicine, Yantai Shandong 264100, China)

机构地区：[1]山东中医药高等专科学校西医教学部,山东烟台264100

出　　处：《中国卫生标准管理》2017年第9期130-131,共2页China Health Standard Management

摘　　要：目的探讨肥胖大鼠心外膜脂肪(EAT)巨噬细胞(M)数量和血清脂肪因子水平。方法 18只SD大鼠随机分为对照组9只和肥胖组9只,分别饲以基础饲料和高脂饲料。80天后采血并取EAT。脂肪标本行CD68抗体标记的免疫组化检测。检测瘦素、脂联素和内脏脂肪素。结果肥胖组M数量高于对照组。与对照组相比,肥胖组脂联素显著降低,而内脏脂肪素、瘦素显著升高。肥胖组M数与内脏脂肪素、瘦素呈正相关,与脂联素呈负相关。结论肥胖大鼠EAT M数增多,并与全身慢性炎症关系密切。Objective To explore the characteristics of macrophage infiltration in epicardial adipose tissue（EAT） and serum fatty cytokine level in obese rats. Methods 18 SD rats were selected and randomly divided into the control group and the obese group. After 80 days, the control group were fed with basal diet and the obese group were fed with high fat diet. EAT were detected by immunohistochemistry with CD68 antibody. Serum leptin, adiponectin and visfatin were detected. Results The number of macrophage in the obese group were significantly higher than the control group. The level of leptin and visfatin in the obese group were significantly higher and adiponectin was significantly lower than the control group. The number of macrophages was negatively correlated with adiponectin and was positively correlated with visfatin and leptin. Conclusion The number of macrophage in EAT in obese rats are increased, and macrophage is closely associated with chronic systemic inflammatory.

关 键 词：肥胖 心外膜脂肪 巨噬细胞 

分 类 号：R589.2[医药卫生—内分泌]