肝癌细胞与肝星状细胞相互作用的实验研究  

Experimental study on the interaction between hepatoma cells and hepatic stellate cells

作　　者：柯萍[1] 官阳[2] 杨木兰[2] 刘冰[2] 周泽斌[2] 张春明[2] 石玉香[2] 吴中杰[3] 

机构地区：[1]嘉兴市第一医院病理科,浙江省嘉兴314000 [2]华中科技大学同济医学院病理学系超微病理研究室,武汉430030 [3]嘉兴市第一医院胸外科,浙江省嘉兴314000

出　　处：《中国医师杂志》2017年第5期687-691,共5页Journal of Chinese Physician

基　　金：浙江省嘉兴市科技计划（2010AY1033,2016AY23040）

摘　　要：目的观察肝癌细胞HepG2与肝星状细胞（HSC）相互作用对二者生长状态的影响，初步探讨肝癌细胞和间质细胞的相互作用在肝癌发展过程中的作用。方法采用MTT、流式细胞仪、免疫组化法、电镜等方法，观察HepG2细胞条件培养基（CM）对HSC增殖、活化及活化HSC细胞CM对HepG2增殖的影响，以及两种细胞直接共培养后的超微结构改变。结果CM能明显促进HSC／HepG2增殖，影响细胞周期；经相应CM作用后，HSC中α-平滑肌肌动蛋白（α—SMA）表达增强，HepG2中增殖细胞核抗原（PCNA）表达增强。直接共培养的肝星状细胞超微结构也显示明显活化改变。结论HepG2细胞能诱导HSC增殖、活化，而HSC也能促进HepG2细胞增殖，两者的相互作用在肝癌的演进过程中可能起重要作用。Objective To investigate the effects of the interaction between human hepatoma cells and hepatic steflate cells on their growth state, and study its role of interaction on the progression of hepato- cellular carcinoma. Methods Human hepatoma cell line HepG2 and hepatic stellate cell line hepatic stallate cells （HSC） -T6 were used and the methods including methyl thiazoiyl tetrazolium （MTT） assay, flow eytometry （FCM） analysis, immunohistoehemistry, and electron microscopy were employed in this experi- ment. The effects of conditioned medium （CM） of HepG2 on the activation and proliferation of HSC were explored. The effects of activated HSC CM on HepG2 proliferation were investigated. The uhrastructural changes of the two eo-euhured cells were observed. Results MTF assay result showed that HepG2/HSC CM could promote HSC/HepG2 proliferation. FCM result demonstrated that HepG2/HSC CM could influ- ence the cell cycle distribution in HSC/HepG2. Immunohistoehemistry exhibited that after the treatment of HepG2/HSC CM, the expression ofc^-smooth muscle actin （c^-SMA） in HSC and proliferating cell nuclear antigen （PCNA） in HepG2 were increased. When HepG2 and HSC were co-cultured, the uhrastrueture of HSC displayed an activated feature. Conclusions HepG2 cells can induce the activation and proliferation of HSC, and the activated HSC can also stimulate the proliferation of HepG2. Interaction between hepatoma cells and hepatic stellate cells may play an important role in the progression of hepatocellular carcinoma.

关 键 词：肝肿瘤 肝细胞 生长 

分 类 号：R735.7[医药卫生—肿瘤]