水曲柳FmABI5基因非生物胁迫及信号诱导的表达模式分析  

Expression Patterns of FmABI5 Gene Induced by Abiotic Stress and Signal in Fraxinus mandshurica

作　　者：孙爽[1] 赵兴堂[1,2] 梁楠松[1,2] 刘颖[1,2] 于磊[1,2] 刘春浩[1] 覃俊祺 詹亚光[1,2] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物研究》2017年第3期453-460,共8页Bulletin of Botanical Research

基　　金：国家自然科学基金面上项目(31270697);国家科技支撑项目(2012BAD21B020106);东北林业大学大学生创新创业训练计划项目(201610225233)~~

摘　　要：ABI5(abscisic acid-insensitive 5)蛋白是一个响应ABA信号的碱性亮氨酸拉链类(basic leucine zipper,b ZIP)转录因子。揭示了水曲柳FmABI5在非生物胁迫下的表达特征,为该基因在水曲柳中代谢调控功能的研究奠定基础。获得了水曲柳ABI5基因的全长,命名为FmABI5,应用生物信息学软件分析了水曲柳FmABI5基因的分子结构特征,利用PEG、低温(4℃)及盐(NaCl)进行非生物胁迫处理,利用脱落酸(ABA)和赤霉素(GA3)进行信号诱导,分析FmABI5的表达特征。生物信息学分析表明该基因全长1 455 bp,含有完整的开放阅读框,编码484个氨基酸。FmABI5为不稳定类亲水性蛋白,不存在信号肽,具有跨膜能力,α-螺旋、延伸链、无规则卷曲分布于整个蛋白。分子进化分析结果表明,水曲柳FmABI5基因与芝麻的遗传距离较近,说明其亲缘关系较近;与马铃薯、潘那利番茄、水茄与甜椒的遗传距离较远,说明其亲缘关系较远。非生物胁迫结果表明,FmABI5基因表达水平随非生物胁迫处理时间不同而上下波动,但在处理6、48和72 h后,FmABI5基因对3种非生物胁迫处理都上调表达,说明FmABI5基因对非生物胁迫具有响应。信号诱导结果表明,外源的ABA与GA共同调节了FmABI5基因的表达。Abscisic acid-insensitive 5 （ABI5） protein was a basic leucine zipper （bZIP） transcription factor containing a basic DNA-binding domain linked to a leucine zipper domain and ABI5 protein response abscisic acid. We studied the FmABI5 expression pattern in signal induction and regulation, and analyzed gene function in metabolic regulation of Fraxinus mandshurica. The ABI5 gene was cloned from F. mandshurica, named FmABIS. The molecular structure of FmABI5 was analyzed by bioinformatics software. Abiotic stress by polyethylene glycol （ PEG）, salt （ NaC1 ） and low temperature （4℃ ）, and signal induced by abscisic acid （ABA） and gibberellin （GA） were used in the study. The expression patterns of FmABI5 induced by abiotic and signal induction were analyzed. By bioinformatics analysis, FmABI5 is 1 455 bp, and contains the complete ORF, encoding 484 amino acids. FmABI5 is a hvdroDhobic protein without signal peptide but with one transmembrane ability. The alpha helix, extension chain and random coil are distributed throughout the protein. By molecular evolution analysis, the genetic distance of FmABI5 gene between F. mandshurica and Sesamum is relative close. The gene heredity distance is farther with Solanum tuberosum, S. pennellii, S. torvum and Capsicum annuum, explaining the far genetic relationship with them. After abiotic stress, FmABI5 gene expression levels varied with abiotic stress time with fluctuations, but after 6, 48 and 72 h treatment, FmABI5 gene for three abiotic stress treatments were up-regulated, indicating FmABI5 genes are responsive to abiotic stresses. Therefore, the signal induced by exogenous ABA and GA regulate transcription expression of FmABI5 together.

关 键 词：水曲柳 FmABI5 生物信息学分析 非生物胁迫 

分 类 号：Q949.776.2[生物学—植物学]