PRRSV强弱毒体外诱导IL-10产生差异的分子基础研究  

MOLECULAR MECHANISM OF DIFFERENTIAL ABILITY FOR IL-10 EXPRESSION INDUCED BY HIGH AND LOW VIRULENT PRRSV IN VITRO

作　　者：夏天奇[1] 姜一峰[1] 虞凌雪[1] 周艳君[1] 杨莘[1] 高飞[1] 李丽薇[1] 曲泽慧[1] 童光志[1,2] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]江苏高校动物重要疫病与人兽共患病防控协同创新中心,扬州225009

出　　处：《中国动物传染病学报》2017年第2期43-48,共6页Chinese Journal of Animal Infectious Diseases

基　　金："十二五"农村领域国家科技支撑计划(2015BAD12B01);国家自然科学基金面上项目(31670158)

摘　　要：猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)感染后能够引发机体产生免疫抑制,IL-10在病毒介导的免疫抑制中起重要作用。PRRSV HuN4株感染PAM细胞后能够诱导IL-10高水平表达,而其传代致弱毒株HuN4-F112感染PAM细胞后IL-10表达水平很低。本研究以PRRSV HuN4株及HuN4-F112株为研究对象,对其感染PAM细胞后IL-10上游关键因子表达差异进行研究,揭示诱导IL-10产生差异的分子基础。结果显示,PRRSV强弱毒感染PAM细胞后,强毒HuN4株诱导TLR2与TLR4转录水平显著高于弱毒HuN4-F112株,TLR3、TLR7和TLR9转录水平则无显著差异;强毒HuN4株感染PAM细胞后,其p38磷酸化水平显著高于弱毒HuN4-F112株,而ERK磷酸化水平差异不具备显著统计学意义。结果提示,PRRSV强弱毒株感染PAM细胞诱导IL-10表达水平的差异推测是由于强弱毒诱导p38 MAPK磷酸化水平差异造成的。Porcine reproductive and respiratory syndrome virus （PRRSV） could induce serious immune suppression after infection, as an important immuno-regulatory cytokine, IL-10 plays an important role in virus mediated immune suppression. Previous study suggested that the IL-10 expression level existed a big difference during PRRSV HuN4-F5 and HuN4-F112 infection, high level of IL-10 was found when PAMs infected with rHuN4-F5, while the level of IL-10 in PAMs infected with rHuN4-F112 was very low. To further investigate the difference of IL-10 expression during PAMs infected with HuN4 or HuN4-F112, we measured key cytokines involved in IL-10 pathway. The results showed that the expression of TLR2 and TLR4 in PAMs infected with HuN4 were significantly higher than that of infected with HuN4-F112, while the expression of TLR3, TLR7, and TLR9 showed no difference between them. The phosphorylation of p38 and ERK were further tested, and significantly higher phosphorylation of p38 was found when PAMs was infected with HuN4 than that of HuN4-F 112. No difference of ERK phosphorylation was found between the two groups. All these results indicated that HuN4 could induce significantly higher IL-10 expression in PAMs than that of HuN4-F 112, the difference expression of IL-10 between HuN4 and HuN4-F112 infection was relative to the different phosphorylation of p38 in MAPK pathway which was beneficial for IL-10 expression.

关 键 词：PRRSV IL-10 PAMs P38 MAPK 

分 类 号：S852.659.5[农业科学—基础兽医学]