数字PCR技术在转基因成分检测中的应用  

Application of Digital PCR Technology in Detection of Genetically Modified Components

作　　者：付海滨[1,2] 闫超杰 李姝[1,2] 刘晓超[2] 张敏[4] 

机构地区：[1]沈阳检验检疫科学研究院,辽宁沈阳110016 [2]沈阳出入境检验检疫局技术中心,辽宁沈阳110016 [3]锦州出入境检验检疫局,辽宁锦州121013 [4]沈阳农业大学,辽宁沈阳110866

出　　处：《辽宁农业科学》2017年第1期50-53,共4页Liaoning Agricultural Sciences

基　　金：沈阳市科技计划项目(F16-104-4-00)

摘　　要：近年来,世界各国都非常重视转基因成分检测技术的研究和应用,因此,建立快速简单、高灵敏度、高精确度的转基因成分检测方法十分必要。数字PCR(digital PCR,d PCR)技术作为一种全新的核酸检测方法,近年来在转基因检测领域备受青睐。文中介绍了数字PCR(digital PCR,d PCR)技术的基本原理,概述了其在转基因成分检测领域的应用进展。In recent years, many countries attach great importance to the research and application of the de-tection technology of genetically modified components . Therefore, it is necessary to establish a rapid, simple, high sensitivity and high precision detection method of transgenic components. Digital PCR （ digital, dPCR） technology as a new nucleic acid detection methods, in recent years in the field of transgenic detection favored by the PCR. This paper introduces the basic principle of digital PCR （ digital, dPCR） technology,and summa-rizes its application in the field of genetically modified component detection （PCR）.

关 键 词：数字PCR 转基因产品 检测方法 

分 类 号：S188[农业科学—农业基础科学]