高效液相色谱法测定云南红药胶囊中的三七皂苷R_1、重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ的含量  

HPLC method for simultaneous determination of notoginsenoside R_1,chonglousaponin Ⅶ,chonglousaponin Ⅵ,chonglousaponin Ⅱ and chonglousaponinⅠ in yunnan hongyao capsules

作　　者：张铭[1] 袁杰[1] 张新艳[2] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032 [2]辽宁省军区沈阳第一干休所卫生所,辽宁沈阳110031

出　　处：《安徽医药》2016年第12期2257-2261,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法(HPLC)同时测定云南红药胶囊中三七皂苷R_1、重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ的含量,为云南红药胶囊质量的全面评价提供了科学依据。方法采用Diamonsil C_(18)柱(250 mm×4.6 mm,5μm),以乙腈(A)-水(B)为流动相,进行梯度洗脱(0~12 min,18.0%A;12~19 min,18.0%A→25.0%A;19~27 min,25.0%A→45.0%A;27~34 min,45.0%A→55.0%A;34~40 min,55.0%A→18.0%A),检测波长为203 nm。流速0.9 m L·min^(-1),柱温30℃,进样量为10μL。结果三七皂苷R_1、重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ5个成分分别在7.32~146.40、4.70~94.00、3.85~77.00、6.97~139.40、11.09~221.80 mg·L^(-1)内与色谱峰峰面积呈良好的线性关系;平均加样回收率均在97.12%~99.86%,RSD≤1.37%(n=6)。结论该研究建立的HPLC法同时测定云南红药胶囊中的5个成分,样品处理方法简便,方法专属性强,测定结果准确,重复性好,为云南红药胶囊质量的全面评价提供了科学依据。Objective To establish an HPLC method for determination of notoginsenoside R1,chonglousaponin Ⅶ,chonglousaponin Ⅵ,chonglousaponin Ⅱ and chonglousaponin Ⅰ,and to provide a scientific basis for the comprehensive quality evaluation of yunnan hongyao capsules.Methods The HPLC method was adopted with the Diamonsil C（18）column（250 mm × 4.6 mm,5 μm）and acetonitrile（A）-water（B）with gradient elution（0 - 12 min,18.0% A; 12 - 19 min,18.0% A→25.0% A; 19 - 27 min,25.0% A→45.0%A; 27 - 34 min,45.0% A→55.0% A; 34 - 40 min,55.0% A→18.0% A）as the mobile phase at the flow rate of 0.9 m L·min^-1.The detection wavelength was 203 nm,the column temperature was set at 30 ℃,and sample quantity was 10 μL.Results The good linear relationships for notoginsenoside R1,chonglousaponin Ⅶ,chonglousaponin Ⅵ,chonglousaponin Ⅱ and chonglousaponin Ⅰwere within the ranges of 7.32 - 146.40,4.70 - 94.00,3.85 - 77.00,6.97 - 139.40,11.09 - 221.80 mg·L^-1,respectively.The average recoveries were between 97.12% and 99.86%,RSD≤1.37%（n = 6）.Conclusions An HPLC with gradient elution method has been successfully established for simultaneous determination of 5 components in yunnan hongyao capsules.The sample processing method is simple,specific,accurate,and repeatable,which provides a scientific basis for the comprehensive quality evaluation of yunnan hongyao capsules.

关 键 词：云南红药胶囊 三七皂苷R1 重楼皂苷Ⅶ 重楼皂苷Ⅵ 重楼皂苷Ⅱ 重楼皂苷Ⅰ 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]