不同移虫日龄蜂王卵巢中hexamerin110、hexamerin70b的差异表达分析  

Analysis on differential expression of hexamerin 110 and hexamerin70b in the ovaries of queens reared from different instar of the grafted larvae

作　　者：庞倩[1] 王莹[1] 王康[1] 张文文[1] 吉挺[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《环境昆虫学报》2017年第1期62-67,共6页Journal of Environmental Entomology

基　　金：国家自然科学基金(31502020;31172272);现代农业产业技术体系(CARS-45-SYZ6)

摘　　要：为探究不同移虫日龄的西方蜜蜂蜂王卵巢中hexamerin110、hexamerin70b的表达及其调控机理,以β-actin为内参基因,运用q RT-PCR技术检测不同移虫日龄蜂王卵巢中hexamerin110、hexamerin70b m RNA的表达水平,用Western blot方法检测hexamerin110、hexamerin70b蛋白的表达。结果显示,hexamerin110、hexamerin70无论是在m RNA水平还是蛋白水平,随着移虫日龄的增加,其表达量均呈降低的趋势。1日龄移虫培育的蜂王卵巢hexamerin110基因表达量显著高于其他组(P<0.05),1日龄移虫培育的蜂王卵巢的hexamerin70b基因的表达量极显著高于其他组(P<0.01)。Hexamerin110、hexamerin70b在1日龄移虫发育的蜂王卵巢中的表达水平最高,推测其可能与蜂王生殖发育以及级型分化有关。To explore the expression and molecular regulation mechanism of hexamerin110 and hexamerin110 in the ovaries of queens reared from different in star of the grafted larv ae, mRNA and protein expression of hexamerin110 and hexamerinh were assessed by qRT-PCR and Western blot. The results showed that the expression levels of the hexamerinl 10 and hexamerinlOh decreased with the increasing instar of the grafted larvae, the expression levels of the hexamerin110 in ovaries of queens reared from 1 day instar of the grafted larvae were significantly higher than others （ P 〈 0. 05 ） . Meanwhile, the expression levels of the hexamerin110 in ovaries of queens reared from 1 day instar of the grafted larvae were extremely significantly higher than others （ P 〈 0. 01 ） . The expression levels of the hexamerin110 and hexamerin110 of 1 day instar of the grafted larvae are the highest, it is speculated that hexamerinl 10 and hexamerin110 may be associated with queen reproductive development and caste differentiation.

关 键 词：移虫日龄 卵巢 hexamerin110 hexamerin70b 级型分化 

分 类 号：Q963[生物学—昆虫学] S89[农业科学—特种经济动物饲养]