棉铃虫对转Bt基因棉花的行为反应  

Behavioral responses of Helicoverpa armigera (Hübner) larvae to transgenic Bt cotton

作　　者：赵冬晓[1,2] 刘标[1] 

机构地区：[1]环境保护部南京环境科学研究所,南京210042 [2]南京大学生命科学学院,南京210023

出　　处：《环境昆虫学报》2017年第1期152-159,共8页Journal of Environmental Entomology

基　　金：转基因生物新品种培育重大专项之"自然生态风险监测与控制技术"重大课题(2016ZX08012-005);公益性环保科研专项课题(201509044);中国博士后科学基金(2015M571664)

摘　　要：棉铃虫Helicoverpa armigera(Hübner)是转Bt基因棉花(Bt棉)最重要的靶标害虫之一。害虫对Bt棉的抗性问题是Bt棉产业持续健康发展的最主要威胁。规避行为可以减少害虫与Bt棉的接触而降低生理抗性选择压力。在本研究中,通过比较棉铃虫对Bt棉与常规棉的行为反应评估了棉铃虫对Bt棉的行为规避能力。产卵选择结果显示,棉铃虫成虫在Bt棉上的落卵量显著低于常规棉。幼虫实验结果显示,棉铃虫初孵幼虫在Bt棉植株上的居留时间显著短于在常规棉上的居留时间。无选择条件下,1龄幼虫在对Bt棉叶的取食空洞数以及总取食量均显著低于常规棉叶。综合以上的结果,认为棉铃虫对Bt棉具有一定的行为规避能力,这可能是延缓棉铃虫对Bt棉产生抗性的因素之一。本研究能够帮助有效地预测靶标害虫对Bt作物的抗性风险。Helicoverpa armigera （ Hubner） is one cotton. The evolution of resistance by pests is theof the most important target pests of transgenic Bt main threat to the continued success of Bt cotton.Behavioral avoidance might lead to reduce exposure to Bt cotton and minimize selection for physiological resistance. In this study, we determined if behavioral avoidance against Bt cotton present in H. armigera by comparing its behavioral responses to Bt and non-Bt cottons. The oviposition choice experiment showedthat the number of eggs on Bt cotton plants were significantly lower than on non-Bt cotton plants. The results of larvae test indicated that the residence time of H. armigera neonates on Bt cotton plants wasmuch shorter than that on conventional cotton plants. Under no-choice conditions, both the number of consumed holes and the total consumed leaf area of first in stars on Bt leaves were significantly less thanthose of first instars on non-Bt leaves. According to the results, we conclude that H. armigera larvae exhibit behavioral avoidance to Bt cotton, providing important insights into one possible mechanism underlying the durability of Bt cotton resistance. This study may be useful for improving strategies predicting the risk of pest resistance to Bt crops.

关 键 词：棉铃虫 BT棉 产卵 取食 规避 

分 类 号：Q968.1[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]