CCK-8法检测九香虫血淋巴对人乳腺癌MCF-7细胞增殖的抑制作用  

The inhibitory effect of haemolymph from Aspongopus chinensis Dallas on growth of human breast cancer MCF-7 by CCK-8

作　　者：杨佳琪[1] 檀军[2] 曹米兰 金道超[1] 郭建军[1] 

机构地区：[1]贵州大学昆虫班究所贵州山地农业病虫害重点实验室,贵阳550025 [2]遵义医学院,贵州遵义563099

出　　处：《环境昆虫学报》2017年第1期193-197,220,共6页Journal of Environmental Entomology

基　　金：国家自然科学基金(81360612);贵州省科学基金省校联合项目(黔科合LH字[2014]7650);贵州省优秀青年科技人才培养对象专项资金项目(黔科合人字[2015]25号);贵州省科技厅创新人才团队建设项目(黔科合人才团队[2014]4001号)

摘　　要：为了明确九香虫血淋巴对人乳腺癌MCF-7细胞增殖的抑制作用,本文利用Bradford法检测九香虫血淋巴浓度,用不同浓度的九香虫血淋巴处理体外培养的MCF-7细胞,采用CCK-8法检测九香虫血淋巴对MCF-7细胞的抑制作用。结果表明:九香虫血淋巴能显著抑制人乳腺癌MCF-7细胞的生长,且对MCF-7的抑制率呈时间、剂量依赖关系。To clear the inhibitory effect on human breast cancer cell line MCF-7 by the haemolymph from Aspongopus chinensis Dallas, 1851,Bradford method was used to test the concentrations of the haemolymph from A. chinensis, the MCF-7 cells cultured in vitro were treated by different concentrations of the haemolymph, and at last the inhibition rates to MCF-7 cells were detected by CCK-8 method. The results showed that the haemolymph from A. chinensis could inhibit proliferation of human breast cancer MCF-7 cells in vitro with dosage- and time-dependent effects.

关 键 词：九香虫 血淋巴 乳腺癌 CCK-8法 

分 类 号：Q965.9[生物学—昆虫学] R273[医药卫生—中西医结合]