聚六亚甲基胍对异育银鲫鳃出血病药效的初步研究  

A pilot study of the effect of polyhexamethylene guanide on Carassius auratus gibelio hematopoietic necrosis

作　　者：赵亮[1] 邹益东 胡鲲[1] 杨先乐[1] 黄保华 

机构地区：[1]上海海洋大学,国家水生动物病原库,上海201306 [2]江苏天石生物科技有限公司,无锡214258 [3]上海黛龙生物科技有限公司,上海201400

出　　处：《淡水渔业》2017年第2期96-100,共5页Freshwater Fisheries

基　　金：国家重大科技成果转化项目(ZD-2012-345);上海高校知识服务平台项目(ZF1206);公益性行业(农业)科研专项经费(201203085);国家863计划项目(2011AA10A216);国家自然科学基金资助项目(31172430)

摘　　要：通过PCR检测及序列对比分析,确诊江苏海丰农场两塘口内异育银鲫(Carassius auratus gibelio)所患疾病为鲤疱疹病毒Ⅱ型(Cyprinid herpesvirusⅡ,Cy HV-2)引发的鲫造血器官坏死症(Crucian CarpHematopoietic Necrosis),而后分别每10 d使用0.5 m L/m^3和0.75 m L/m^3聚六亚甲基胍(Polyhexamethylene guanide,PHMG)泼洒治疗,并定期采样,利用Real Time PCR测定样品中病毒表达量。结果显示:该病毒(DF2015)Cy HV-2的DNA解旋酶基因序列长为316 bp,与绝大多数病毒株有较近的亲缘关系(99%),但与病毒株ST-J1和H.Fukuda的亲缘关系相对较远(63%)。与其余病毒株等有较近的亲缘关系(99%);治疗近2个月后,两塘的病毒相对表达量均呈下降趋势,且具有显著差异性,但0.75 m L/m^3治疗塘病毒相对表达量极显著降低,治疗效果相对更显著。Using nested - PCR assay and the amplification product assay, we decided the disease of Carassius auratus gibe -lio in two areas of Jiangsu Haifeng Farm is caused by Cyprinid herpesvirus II （ CyHV - 2） , and then respectively treated with 0. 5 mL/m3 and 0. 75 mL/m3 Polyhexamethylene guanide （PHMG） each 10 days, sampled regularly, and eventually evaluated the relative expression of CyHV -2 by Real Time PCR. The results showed that the sequence of vims ’ CyHV - 2 DNA helicase gene is 316bp, and forms an independent branch on the phylogenetic tree with other CyHV - 2 strains. Af-ter two months treatment, relative expression of CyHV - 2 of both treatments decreased significantly, and relative expression of CyHV -2 of the one treating with 0. 75 mL/m3 PHMG decreased more significantly, and the efficiency is much better.

关 键 词：聚六亚甲基胍 异育银鲫(Carassius auratus gibelio) 鲫造血器官坏死症 实时荧光定量PCR 鲤疱疹病毒Ⅱ型 

分 类 号：S948[农业科学—水产养殖]