蔺草种质资源遗传多样性的ISSR分析  

Genetic diversity of Juncus effusus germplasm by ISSR markers

作　　者：柴晓娟[1] 郭玮龙 陈慧[1] 王俊荣[1] 谢德志[1] 金水虎[1] CHAI Xiaojuan GUO Weilong CHEN Hui WANG Junrong XIE Dezhi JIN Shuihu(School of Forestry and Biotechnology, Zhejiang A ＆ F University, Lin＆an 311300, Zhejiang, China Agriculture and Forestry Bureau of Shengzhou City, Shengzhou 312400, Zhejiang, China)

机构地区：[1]浙江农林大学林业与生物技术学院,浙江临安311300 [2]浙江省嵊州市农林局,浙江嵊州312400

出　　处：《浙江农林大学学报》2017年第3期552-558,共7页Journal of Zhejiang A&F University

基　　金：浙江省宁波市科技资助项目(2012C10039)

摘　　要：为研究蔺草Juncus effusus种质资源遗传多样性,采用简单序列重复区间扩增多态性(inter-simple sequence repeats,ISSR)分子标记技术对来自不同区域的36份蔺草种质资源进行遗传多样性分析,并探讨它们的亲缘关系。结果显示:72对ISSR引物中共筛选出23对引物能扩增出稳定清晰条带;36份蔺草种质的基因组DNA共扩增出148条带,其中130条多态性条带,多态性比率达87.84%。应用NTSYSpc 2.1软件分析显示,36份蔺草种质材料间的遗传相似系数(genetic similarity,GS)为0.405 6~0.944 1,表明蔺草种质资源具有丰富的遗传多样性。非加权组平均法(unweighted pair group method using arithmetic averages,UPGMA)聚类显示,在遗传相似系数0.650 3处可将36份蔺草种质划分为5大类群,第Ⅰ大类群可分为3个亚类,大多数来自于相同或相近地理区域的材料聚于同一类或亚类,野生种质与栽培种质有分别聚在一起的趋势。研究结果从分子水平揭示了蔺草种质资源的主要遗传多态性特点,为今后蔺草育种及杂交亲本的选择提供理论依据。To study the genetic diversity of Juncus effusus resources, the genetic diversity of 36J . effusus germplasm resources from both home and abroad were analyzed by Inter Simple Sequence Repeats （ISSR） markers. Analysis included an Unweighted Pair Group Method with Arithmet ic Mean （UPGMA） cluster analy-sis. Results showed 23 primers with clear polymorphic bands selected from 72 primers. A total of 148 bands, of which 130 were polymorphic, were amplified from the 36 materials with the proportion of polymorphic loci be-ing 87.84%. The genetic s imilarity coefficient for varieties was between 0.405 6 and 0.944 1, which showed rich genetic diversity. The UPGMA cluster analysis produced a genetic similarity coefficient of 0.650 3 for the boundaries with the 36 materials divided into five groups and the firs t group being divided into three sub-groups. Results were connected to region and cultivar origin with materials from the same region mostly classi-fied into the same group or subgroup. This research revealed the genetic diversity of J . effusus germplasm re-sources at the molecular level for the f irs t time which could provide a theoretical basis for breeding.

关 键 词：植物学 蔺草 种质资源 ISSR标记 遗传多样性 

分 类 号：S718.46[农业科学—林学]