分子探针99Tcm－Duramycin和99Tcm－RGD用于动脉易损斑块分子显像的对比研究  

Comparative study of arterial vulnerable plaque molecular imaging with novel molecular probes 99Tcm-Duramycin and 99Tcm-RGD in rabbit models

作　　者：张颖[1] 牟甜甜[1] 王蒨[1] 韩洁[1] 苏航[1] 谷姗姗[1] 

机构地区：[1]首都医科大学附属北京安贞医院核医学科,100029

出　　处：《中华核医学与分子影像杂志》2017年第5期289-293,共5页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：国家自然科学基金（81271613）

摘　　要：的比较99Tcm－Duramycin和99Tcm－RGD对模型家兔动脉粥样硬化斑块探测的可行性及效能。方法选取15只健康新西兰雄性大白兔，完全随机法分为3组：普食对照组（A组，5只）、稳定斑块组（B组，5只）和易损斑块组（C组，5只），分别于饲养4、8、12周末注射99Tcm－Duramycin和99Tcm－RGD，并于注射后0.5 h行SPECT/CT显像。4、8周末各组分别处死1只，12周末显像结束后全部处死。离体观察C组动物的腹主动脉；8、12周末显像前行血管内超声（IVUS）检查对照。采用配对t检验分析数据。结果2个探针在A组3个时间点的显像中，腹主动脉区域几乎无放射性浓聚；在B组8周和12周末的显像中，腹主动脉处均有一定摄取，但99Tcm－Duramycin的放射性分布较99Tcm－RGD略高；在C组腹主动脉处的摄取明显高于同期的A、B组，且99Tcm－Duramycin明显高于99Tcm－RGD。C组中99Tcm－Duramycin和99Tcm－RGD在4周末T/NT比值分别为2.14±0.34和1.46±0.34，8周末分别为2.93±0.41和1.66±0.22，12周末分别为3.25±0.29和1.81±0.28，差异均有统计学意义（t值：4.072、5.578、19.692，均P〈0.05）。12周末C组腹主动脉内膜见明显凸出于管腔的黄色脂质斑块；IVUS检查显示，C组8周、12周末内膜厚度、管腔狭窄率分别为（450±104）和（767±52） mm，（29.30±2.81）%和（37.98±6.41）%，差异均有统计学意义（t值：44.024和9.226，均P〈0.05）。结论99Tcm－Duramycin和99Tcm－RGD均可用于早期动脉易损斑块显像。99Tcm－Duramycin可先于99Tcm－RGD探测到斑块，且可提供信噪比更高的图像，更有助于得到易损斑块的相关信息。ObjectiveTo investigate the potential of 99Tcm-Duramycin and 99Tcm-RGD in detecting vulnerable plaque in rabbit models.MethodsFifteen healthy New Zealand male rabbits were randomly divided into group A （control group, n=5）, group B （stable plaque group, n=5） and group C （vulnerable plaque group, n=5）. Animals were injected with 99Tcm-Duramycin and 99Tcm-RGD at the end of 4, 8 and 12 weeks. SPECT/CT scanning was performed at 0.5 h post injection. One rabbit was sacrificed at the end of 4 weeks and one at the end of 8 weeks after imaging. The others were sacrificed at the end of 12 weeks after imaging studies. All aortas were collected. Intravascular ultrasound （IVUS） was performed at the end of 8, 12 weeks before SPECT/CT scanning. The data was analyzed with paired t test.ResultsIn group A, the aortas had little uptake of the two probes. In group B, the aortas showed obvious radioactive uptake of 99Tcm-Duramycin and 99Tcm-RGD at the end of 8 weeks and 12 weeks, while 99Tcm-Duramycin gave better display than 99Tcm-RGD. In group C, 99Tcm-Duramycin uptake was higher than 99Tcm-RGD uptake in the aorta. The T/NT ratios of 99Tcm-Duramycin and 99Tcm-RGD in group C were 2.14±0.34 and 1.46±0.34 （t=4.072, P〈0.05） at the end of 4 weeks, 2.93±0.41 and 1.66±0.22 （t=5.578, P〈0.05） at the end of 8 weeks, 3.25±0.29 and 1.81±0.28 （t=19.692, P〈0.05） at the end of 12 weeks. In isolated specimen of group C, the yellow lipid plaque of the intima bulged on the lumen at the end of 12 weeks. IVUS indicated that, at the end of 8 weeks and 12 weeks, the endometrial thickness of group C was （450±104） mm and （767±52） mm （t=44.024, P〈0.05） respectively, and the rates of luminal stenosis were （29.30±2.81）% and （37.98±6.41）% （t=9.226, P〈0.05）.ConclusionsBoth 99Tcm-Duramycin and 99Tcm-RGD may be used to detect vulnerable plaque at early time. 99Tcm-Duramycin may detect vulnerable atherosclerotic plaque earlier than 99Tcm-RGD and provide better diagnostic image.

关 键 词：动脉粥样硬化性 分子成像 体层摄影术 发射型计算机 单光子 体层摄影术 x线计算机 超声检查 介入性 精氨酸-甘氨酸-天冬氨酸 Duramycin 兔 

分 类 号：R54[医药卫生—心血管疾病] R817.4[医药卫生—内科学]