HPLC法同时测定抗骨增生丸中毛蕊花糖苷、柚皮苷和淫羊藿苷的含量  

Simultaneous determination of verbascoside,naringin and icariin in Kangguzengsheng pills by HPLC

作　　者：朱燕 黄义纯 严晓明 

机构地区：[1]韶关市食品药品检验所,韶关512028

出　　处：《药物分析杂志》2017年第5期917-921,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定抗骨增生丸中毛蕊花糖苷、柚皮苷和淫羊藿苷3种成分含量的高效液相色谱法。方法:应用HPLC-梯度洗脱法,采用C_(18)色谱柱(250 mm×4.6 mm,5μm),以水为流动相A,甲醇为流动相B,乙腈为流动相C进行梯度洗脱,流速1.0 mL·min^(-1),检测波长270 nm,柱温25℃。结果:毛蕊花糖苷、柚皮苷和淫羊藿苷测定浓度的线性范围分别为5.08~101.6、7.56~151.2和5.13~102.6μg·mL^(-1),r均大于0.999;平均加样回收率分别为100.5%、99.5%和99.3%,RSD均小于1.0%。样品中毛蕊花糖苷、柚皮苷、淫羊藿苷含量分别为1.06~1.17、1.75~1.88、1.32~1.53 mg·丸^(-1)。结论:该实验方法可有效地控制抗骨增生丸的质量。Simultaneous determination of verbascoside, naringin and icariin ） in icariin in Kangguzengsheng pills by HPLC ZHU Yan, HUANG Yi-chun, YAN Xiao-ming （ Shaoguan Institutes for Food and Drug Control, Shaoguan 512028, China ） Objective： To establish an HPLC method for determining three constituents （ verbascoside, naringin and Kangguzengsheng pills. Methods： The separation was performed on a Cls column （ 250 mm × 4.6 mm, 5 μm ） with gradient elution. The mobile phase was composed of water solution （ A ）, methanol （ B ） and acetonitrile （ C ） at the flow rate of 1.0 mL· min^-1. The detection wavelength was set at 270 nm and the column temperature was 25 ℃. Results： The linear ranges were 5.08-101.6μg·mL^-1 for verbascoside, 7.56-151.2μg·mL^-1for naringin and 5.13-102.6 μg·mL^-1 for icariin, respectively. The correlation coefficients of all curves were above 0.999. The average recoveries of three analytes were 100.5% for verbascoside, 99.5% for naringin, and 99.3% for icariin, with RSD less than 1.0%. The contents of verbascoside, naringin and icariin in 3 samples were 1.06-1.17 mg per pill, 1.75-1.88 mg per pill and 1.32-1.53 mg per pill, respectively. Conclusion： The developed HPLC method can be used for the quality control of Kangguze

关 键 词：抗骨增生丸 毛蕊花糖苷 柚皮苷 淫羊藿苷 中成药含量测定 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]