基于近红外光谱的银杏叶提取液总黄酮醇苷快速检测研究  

Rapid determination of total flavone glycosides in extraction of ginkgo leaf based on near-infrared spectroscopy

作　　者：吕尚[1] 周海滨 汪俊 饶毅[1] 杨世林[1] 

机构地区：[1]中药固体制剂制造技术国家工程研究中心,南昌330006 [2]宁波立华制药有限公司,宁波315174

出　　处：《药物分析杂志》2017年第5期927-933,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：中管局中医药行业科研专项:中成药生产投料饮片质量均一性控制技术研究(201307009)

摘　　要：目的:研究银杏叶提取过程中总黄酮醇苷的快速检测方法。方法:近红外光谱技术与高效液相色谱法结合,采用偏最小二乘法建立银杏叶提取过程中槲皮素、山柰素、异鼠李素指标的定量校正模型。结果:槲皮素、山柰素、异鼠李素的定量校正模型相关系数(r)分别达到0.992 4、0.998 4和0.985 0,交叉验证均方差(RMSECV)分别为0.062 4、0.050 9和0.024 1,定量校正模型性能稳定,预测效果良好。结论:近红外光谱透射技术可以有效反映银杏叶提取过程总黄酮醇苷含量变化信息,可用于银杏叶提取浓缩过程的快速检测。Objective： To develop a rapid method for determination of the total flavone glycosides during the extraction process of ginkgo leaf. Method： Near-infrared spectroscopy combined with HPLC was applied to establish partial least squares models for determination of quercetin, kaempferide and isorhanmetin during the extraction process. Results： The correlation coefficients （ r ） of quantitative calibration models for quercetin, kaempferide and isorhamnetin were 0.992 4, 0.998 4 and 0.985 0, respectively. And the values of root mean square error of cross validation （ RMSECV ） were 0.062 4, 0.050 9 and 0.024 1, respectively. The models performed stable with good predictability. Conclusion： Near-infrared spectroscopy could reflect the variation of total flavone glycosides during the extraction process, and could be used in rapid analysis of the extraction and concentration process of ginkgo leaf.

关 键 词：银杏叶 近红外光谱 提取过程 总黄酮醇苷 槲皮素 山柰素 异鼠李素 快速检测 

分 类 号：R917[医药卫生—药物分析学]