基于谱效相关的何首乌抗血小板聚集药效物质研究  

Antiplatelet aggregation bioactivity of Polygoni Multiflori Radix with chemical fingerprints and spectrum-effect correlation analysis

作　　者：何琴[1,2] 涂灿[2,3] 王伽伯[2] 刘振杰[2,3] 沙孟晨 张乐[2] 李春雨[2,4] 肖小河[2] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]中国人民解放军第三0二医院全军中医药研究所,北京100039 [3]成都中医药大学药学院,四川成都610000 [4]中国医学科学院药用植物研究所,北京100094

出　　处：《中国中药杂志》2017年第9期1679-1684,共6页China Journal of Chinese Materia Medica

基　　金：国家公益性行业专项(201507002);国家自然科学基金项目(81274026);北京市科技新星计划项目(Z16111000490000)

摘　　要：应用体外抗血小板聚集生物活性检测方法,并结合化学指纹图谱进行谱效相关分析,探索何首乌抗血小板聚集的活性物质。采用体外抗血小板聚集法进行检测,何首乌50%乙醇提取物的抗血小板聚集作用强于10%或90%的乙醇提取物,超声提取优于回流提取。对正交试验的不同提取物进行抗血小板聚集活性评价,抑制率在32.03%~74.56%。谱效相关分析结果表明,反式二苯乙烯苷、顺式二苯乙烯苷、儿茶素与抗血小板聚集活性的相关系数较高且具有统计学意义,分别为0.963(P<0.01),0.902(P<0.01)和0.656(P<0.05);进一步对上述3种与活性相关系数较高化合物的单体进行活性验证,均有较好的抗血小板聚集活性;最后根据3种单体化合物在何首乌中的含量差异计算相对活性贡献度可知,反式二苯乙烯苷是何首乌体外抗血小板聚集的主要活性物质。To explore the active substance of antiplatelet aggregation of Polygoni Multiflori Radix by using chemical fingerprints and antiplatelet aggregation bioactivity test for spectrum-effect correlation analysis. The Polygoni Multiflori Radix was tested by antiplatelet aggregation in vitro,and the results showed that 50% aqueous ethanol extract of Polygoni Multiflori Radix had more potent antiplatelet aggregation effect than 10% or 90% aqueous ethanol extract,and ultrasonic extraction was superior to refluxing extraction in the aspect of antiplatelet aggregation. The antiplatelet aggregation bioactivity of the different Polygoni Multiflori Radix extracts was evaluated and the results showed that the inhibition rate was 32. 03%-74. 56%. Spectrum-effect correlation analysis indicated that trans-stilbene glucoside,cis-stilbene glucoside and catechinic acid had higher correlation coefficient and they were 0. 963（ P〈0. 01）,0. 902（ P〈0. 01） and 0. 656（ P〈0. 05） respectively; furthermore,all of the above three compounds demonstrated significant antiplatelet aggregation bioactivities. Considering their content difference in Polygoni Multiflori Radix,we calculated the relative active contributions,and the results suggested that trans-stilbene glucoside was the main active substance of Polygoni Multiflori Radix in the aspect of antiplatelet aggregation in vitro.

关 键 词：何首乌 抗血小板聚集 化学指纹图谱 谱效相关 二苯乙烯苷 

分 类 号：R284[医药卫生—中药学]