广藿香内生真菌Cerrena sp.中倍半萜类成分及其细胞毒活性研究  

Sesquiterpenoids of endophytic fungus Cerrena sp. from Pogostemon cablin and their cytotoxic activities

作　　者：谢佳倩 陈玉婵[2] 孙章华[2] 刘洪新[2] 李赛妮 李浩华[2] 高晓霞[1] 章卫民[2] 

机构地区：[1]广东药科大学,广东广州510006 [2]广东省微生物研究所省部共建华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室,广东广州510070

出　　处：《中国中药杂志》2017年第9期1693-1698,共6页China Journal of Chinese Materia Medica

基　　金：国家973前期专项(2014CB460613);国家自然科学青年基金项目(31600271);广东省科技计划项目(2015A030302060;2014A030304050);广东省自然科学基金项目(2015A030313710)

摘　　要：为了研究广藿香内生真菌Cerrena sp.A593的次级代谢产物及其细胞毒活性,采用正、反相硅胶柱、Sephadex LH-20凝胶柱、HPLC等色谱方法从菌株A593发酵液中分离得到8个倍半萜类化合物,经波谱数据分析,分别鉴定为chloriolin B(1),chloriolin C(2),pleurocybellone A(3),dihydrohypnophilin(4),cucumin F(5),antrodin A(6),10α-hydroxyamorphan-4-en-3-one(7),connatusin A(8),所有化合物均为首次从Cerrena属真菌中分离得到。体外细胞毒活性结果显示:化合物1~3对Hep G-2,SF-268,MCF-7,NCI-H460 4种肿瘤细胞株具有抑制活性,其IC50在20.33~63.13μmol·L^(-1)。The secondary metabolites of endophytic fungus Cerrena sp. 3-593 from Pogostemon cablin and their cytotoxic activities were investigated. Eight sesquiterpenoids were isolated from the fermentation broth of the strain A593 by silica gel, reverse phase silica gel, Sephadex LH-20, HPLC and so on. Their structures were identified as chloriolin B （ 1 ） , chloriolin C （2）, pleurocybellone A （3）, dihydrohypnophilin （4）, cucumin F（5）, antrodin A （6）, 10a-hydroxyamorphan-4-en-3-one （ 7）, and connatusin A （8）. Compounds 1- 8 were firstly found from the genus Cerrena. All isolated sesquiterpenoids were evaluated for in vitro cytotoxic activities against HepG-2, SF-268, MCF-7 and NCI-H460 tumor cell lines. Compounds 1-3 showed inhibitory activities against the four tumor cell lines with ICsovalues ranging from 20. 33 to 63. 13 μmol · L^-1.

关 键 词：Cerrena sp. 内生真菌 广藿香 倍半萜 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]