纳米银对潮土玉米根际真菌群落结构和多样性的影响  

Effects of silver nanoparticles on fluvo-aquic soil fungal community structure and diversity in maize rhizosphere

作　　者：曹际玲[1,2,3] 冯有智[1,2] 林先贵[1,2] 

机构地区：[1]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,江苏南京210008 [2]中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,江苏南京210008 [3]中国科学院大学,北京100049

出　　处：《菌物学报》2017年第5期633-641,共9页Mycosystema

基　　金：国家自然科学基金(41371255;41301267;41271256)~~

摘　　要：根际真菌是土壤生态系统的重要组成部分,本研究采用土壤盆栽方法,以纳米银(silver nanoparticles,Ag NPs)为研究对象,利用Illumina高通量测序技术对不同Ag NPs施加水平下(0.025、0.25、2.5mg/kg)潮土玉米根际真菌群落结构进行分析。结果表明,潮土玉米根际土壤真菌群落主要由子囊菌门Ascomycota、担子菌门Basidiomycota、芽枝菌门Blastodimycota、壶菌门Chytridiomycota、球囊菌门Glomeromycota和接合菌门Zygomycota等组成,其中以子囊菌门真菌为优势类群。Ag NPs在2.5mg/kg施加水平下显著降低了(P<0.05)玉米根际土壤溶解性有机碳(DOC)含量,改变了根际土壤真菌群落结构,使真菌群落结构发生显著分异(P<0.05),主要表现为降低了根霉菌属Rhizopus、镰刀菌属Fusarium、被孢霉属Mortierella等的相对丰度。相关性分析表明土壤DOC含量的变化与Ag NPs处理下根际土壤真菌群落结构分异存在显著的(P<0.05)相关性。Rhizosphere fungi play an important role in soil ecosystems. In this study, a pot experiment was established to nvestigate the responses of fluvo-aquic soil fungal assemblages in maize rhizosphere to different application level of silver nanoparticles （AgNPs） （0.025, 0.25, 2.5mg/kg） using Illumina sequencing analysis method. The sequencing data revealed that fluvo-aquic soil fungal community in maize rhizosphere was dominated by Ascomycota, followed by Basidiomycota, Blastodimycota, Chytridiomycota, Glomermycota and Zygomycota. AgNPs at 2.5mg/kg greatly shifted the AM fungal community composition, showing as decreased proportion of Rhizopus, Fusarium and Mortierella. It was found that the values of soil dissolved organic carbon （DOC） contents were significantly decreased （P〈0.05） under the high （2.5mg/kg） application level of AgNPs. Statistical analysis revealed that changes in fungal community composition and diversity were strongly （P〈0.05） correlated with changes in soil DOC contents induced by AgNPs.

关 键 词：根际真菌 群落结构 丰度 溶解性有机碳 高通量测序 

分 类 号：S154.3[农业科学—土壤学]