产黄青霉菌响应苯氧乙酸的Ca^(2+)信号转导机制  

Calcium-associated signals responses of Penicillium chrysogenum to phenoxyacetic acid

作　　者：完莉萍[1] 程景胜[1] 李小兵[2] 元英进[1] 

机构地区：[1]天津大学(北洋园校区)化工学院制药工程系,天津300350 [2]石家庄学院生物工程系,河北石家庄050035

出　　处：《微生物学通报》2017年第5期1001-1008,共8页Microbiology China

基　　金：国家重点基础研究发展规划项目(973计划)(No.2014CB745100);国家自然科学基金项目(No.21176183;21576201)~~

摘　　要：【目的】研究青霉素V生产过程中—Ca^(2+)信号转导途径参与产黄青霉菌对外源侧链前体苯氧乙酸的应答机制。【方法】考察4种不同机制的Ca^(2+)信号干扰剂[利心平、乙二醇双(2-氨基乙基醚)四乙酸、苏拉明和硫酸新霉素]对青霉素V产量和产黄青霉菌生物量的影响。运用Fluo-3/AM荧光染料对细胞进行染色,通过荧光显微镜成像和酶标仪定量检测两种方法监测胞内Ca^(2+)浓度的变化。【结果】苯氧乙酸添加后胞内Ca^(2+)相对含量高于对照组49.86%,而1 mmol/L磷酸酯酶C底物抑制剂硫酸新霉素的添加使得胞内Ca^(2+)相对含量降低了53.31%,同时青霉素V产量降低78.71%,表明产黄青霉菌可通过肌醇1,4,5-三磷酸信号途径调节胞内Ca^(2+)浓度来响应苯氧乙酸的胁迫。【结论】首次探究了Ca^(2+)信号转导途径在产黄青霉菌对苯氧乙酸应答中的作用,为丝状真菌中Ins(1,4,5)P3-Ca^(2+)信号转导途径的研究提供理论依据。[Objective] We studied calcium-associated signal responses of Penicillium chrysogenum to phenoxyacetic acid. [Metbods] The effects of different types of Ca2＋ interference agents （Nifedipine, EGTA, Suramin and Neomycin sulfate） for penicillin V production were studied. Intracellular Ca2＋ concentration was measured by Olympus System microscope and SpectraMax M2 Fluorescence Microplate Reader. [Results] Levels of intracellular Ca2＋ in P chrysogenum significantly increased （49.86% higher than the control） at 25 h after treatment with phenoxyacetic acid （POA）, whereas significantly reduced （53.31% lower than the control） after treatment with neomycin sulfate as an intracellular calcium inhibitor. Results indicate that cells regulated intracellular calcium level though Ins（1,4,5）P3 signaling pathways to response POA stress. [Conclusion] Understanding the effect of Ca2＋ signal transduction pathway how P. chrysogenum responses to POA stress provides guidance for industrial penicillin production.

关 键 词：Ca2+信号转导 苯氧乙酸 青霉素 产黄青霉菌 

分 类 号：Q936[生物学—微生物学]