紫花苜蓿种子活力测定方法的比较  

Comparative study of seed vigor test in Medicago sativa

作　　者：潘佳[1] 李荣[1] 郭耘欣 胡小文[1] 

机构地区：[1]兰州大学草地农业科技学院,草地农业生态系统国家重点实验室,甘肃兰州730020

出　　处：《草业科学》2017年第5期1042-1048,共7页Pratacultural Science

基　　金：公益性行业(农业)科研专项(201403048-3);甘肃省重大科技攻关项目(2013GS 05907)

摘　　要：采用标准发芽法、胚根突出法、低温发芽法、电导率法对紫花苜蓿(Medicago sativa)10个种子样品的种子活力进行了测定,结果表明,基于标准发芽法的发芽率和活力指数,可将10个种子样品的种子质量分为3级;基于胚根突出法中胚根突出数可将其分为6级,计数胚根突出数的最适时间为24和28h;基于低温发芽法的活力指数和发芽势可将其分为6级;而电导率法可将其分为7级。因而,电导率法作为紫花苜蓿种子活力评价指标最为敏感,其次为胚根突出法和低温发芽法,标准发芽法最不敏感。Seed vigor of 10 seed samples in Medicago sativa was determined by standard germination test, just germination test, cold germination test, and electrical conductivity test. We found that the l0 seed samples fell into three classification levels based on germination percentage and vigor index in the standard germination test. Under the just germination test, the 10 seed samples could be classified into 6 different levels based on radicle emergence with count time from 24 to 28 h after the beginning of incubation. Under the cold germination test, the seed samples could also be classified into 6 different levels based on vigor index and germination potential. Under the electrical conductivity test, the seed samples could be classified into 7 different levels. Thus, the e- lectrical conductivity test was the most sensitive method for M. sativa seed vigor evaluation, followed by just germination test and cold germination test. The standard germination test was not suitable for seed vigor evaluation.

关 键 词：紫花苜蓿 种子活力 标准发芽法 胚根突出法 低温发芽法 电导率法 

分 类 号：S541.9[农业科学—作物学]