免疫组化检测伪狂犬病毒感染大鼠后在其组织中分布规律的研究  

Immunohistochemical detection of viral distribution in rats experimentally infected with pseudorabies virus

作　　者：樊毅[1] 刘洪亮[1] 李萍[1] 黄剑波[1] 杨凡[1] 姜子义[1] 赵军[1] 许思遥 邓益超 殷玥[1] 毛汐语 吕雯婷[1] 杨泽晓[1,2] 徐志文[1,2] 朱玲[1,2] 

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]四川农业大学动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《中国预防兽医学报》2017年第5期342-345,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：四川省科技支撑计划(2014NZ0043;2017NZ0038);国家"十二五"科技支撑计划项目(2015BAD12B04);长江学者与创新团队发展计划(IRT13083)

摘　　要：伪狂犬病毒(PRV)能够感染神经元细胞并且跨突触传播,该特性使它成为了一种新兴的神经示踪病毒。为探究PRV在动物体内分布情况,本研究构建了PRV人工感染大鼠模型,通过免疫组化技术检测了PRV在大鼠体内各组织器官中的增殖分布情况,同时还将免疫组化检测结果与PCR检测结果相比较。结果表明利用免疫组化技术分别在PRV感染72 h、78 h、84 h、96 h、104 h、108 h后开始依次在大鼠的脑、肺、肾、脾、肝、心检测到PRV。同时在总计36个PRV检测样品中,PCR检测的检出率为50%,免疫组化检测的检出率为36%,两者的符合率为72%。本研究利用免疫组化技术观察PRV感染大鼠后在体内增殖分布规律和病变程度,为进一步探索RV的组织嗜性以及机体抗病毒作用机制奠定基础。Pseudorabies virus （PRV） has the ability to infect neuronal cells and synaptic transmission, which could be applied as a kind of new tracer for neuronal cell studies. To determinate the PRV distribution in artificially infected mouse, we established a PRV-infected rat model to analyze the viral distributionin infected animals. The distribution of PRV in various tissues of infected rats was detected by immunehistochemistry （IHC） assay and was compared with the conventional PCR.The results showed that the brain, lung, spleen and kidney of infected rats could be detected by 1HC assay at 72, 78, 84, 96, 104, 108 hrs post infection. And the detected rates were 50% by PCR and 36% by IHC, respectively, and the coincidence rate of the two assays was 72%. This study provided a basis for further study on the characteristics of PRV and antiviral mechanism of the host.

关 键 词：伪狂犬病病毒 大鼠模型 免疫组化 

分 类 号：S852.65[农业科学—基础兽医学]