哮喘患儿调节性B细胞改变及意义初探  

Changes of Regulatory B Cells in Patients with Acute Asthma and Its Significance

作　　者：马卓娅[1] 张建平[1] 刘萍[1] 王国兵[1] 罗颖[1] 郑跃杰[1] 

机构地区：[1]广东省深圳市儿童医院,广东深圳518026

出　　处：《中国医学创新》2017年第14期12-16,共5页Medical Innovation of China

基　　金：国家自然科学基金资助项目(81102227);深圳市知识创新计划项目(JCYJ 20150403100317068)

摘　　要：目的:探讨调节性B细胞(Regulatory B cells,Breg)在儿童哮喘发病中可能的作用。方法:选取急性期哮喘患儿23例(试验组),同龄健康儿童21例(正常对照组),取血备检。采用流式细胞术分别检测外周血调节性T细胞(Regulatory T cells,Treg)、Breg比例及Breg细胞表面CD80、CD86的表达水平;PCR检测CD4^+T细胞中IL-17A、IL-17F IFN-γ、T-bet、GATA3、RORγt、IL-10、TGF-βm RNA表达;ELISA检测B细胞培养上清中IL-10浓度。结果:(1)试验组患儿急性发作期外周血中CD24^(hi)CD27^+Breg细胞比例及B细胞培养上清的IL-10浓度均比正常对照组低(P<0.05);CD80、CD86表达水平均低于正常对照组(P<0.05)。(2)试验组患儿CD4^+CD25^+Foxp3^+Treg比例低于正常对照组(P<0.05);CD4^+T细胞中T-bet、IFNG、RORγt、IL-17A、IL-17F表达上调,差异均有统计学意义(P<0.05);GATA3表达差异无统计学意义(P>0.05)。(3)CD19^+B细胞中IL-10 m RNA在试验组和对照组差异无统计学意义(P>0.05),而TGF-βm RNA在试验组患儿中升高,差异有统计学意义(P<0.05)。结论:Breg细胞数量及功能异常可能是哮喘发病机制之一。Objective： To investigate the role of regulatory B cells in the pathogenesis in patients with asthma in children.Method： Twenty-three children with acute asthma （ the experiment group ） and twenty-one age-matched healthy subjects （ the healthy control group ） were enrolled in this study.Blood samples were collected and the proportions of Breg and CD4^＋CD25^＋Foxp3^＋regulatory T ceils （ Treg ）, and the expressions of co-stimulatory molecules （ CD80, CD86 ） were analyzed by flow cytometry.The concentration of IL-IO protein was measured by enzyme-linkedimmunosorbent assay.Real-time PCR was performed to evalevaluate the expressions of T-bet, IFNG, ROR γt, IL-17A, IL-17F in CD4^＋ T ceils and IL-IO and TGF- β in CD19^＋B ceils at mRNA level. Result： （ 1 ） The proportions of CD244^hiCD27^＋Breg in the experiment group were significantly found to be lower than those of the healthy control group （ P〈0.05 ）, and its expression levels of CD80 and CD86 were down-regulated （ P〈0.05 ）, the concentrations of IL-10 protein in culture supernatant of B cells from patients were still lower than those of the healthy control group （ P〈0.05 ）. （ 2 ） The proportions of CD4^＋CD25^＋Foxp3^＋Treg was significantly decreased in the experiment group compared with those of the healthy control group （ P〈0.05 ）, and the expressions of T-bet, IFNG, ROR γt, IL-17A, IL-17F at mRNA level were significantly increased inpatients compared with those of the healthy control group （ P〈0.05 ） . （ 3 ） There no significant difference in the expressions of IL-10 at mRNA level in CD19^＋B cells between two groups and the expressions of TGF-β at mRNA level in CD19^＋B cells from patients were higher than those of the healthy control group （ P〈0.05 ） .Conclusion： Breg cell deficiency and its impaired functiom might be one of the important factors causing immune dysfunction in patients with acute asthma.

关 键 词：哮喘 儿童 调节性B细胞 调节性T细胞 白介素-10 

分 类 号：R725.6[医药卫生—儿科]