不同相对湿度对间歇性26℃环境下肉鸡盲肠菌群多样性的影响  

Effects of Different Relative Humidity on Cecal Microflora Diversity of Broilers under Intermittent 26 ℃ Environment

作　　者：彭骞骞 周莹[1] 王雪敏[2] 冯京海[1] 甄龙[1,2] 张少帅[1] 常玉[1] 石玉祥[2] 张敏红[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193 [2]河北工程大学农学院,邯郸056021

出　　处：《动物营养学报》2017年第5期1527-1534,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家科技支撑计划课题"畜禽健康养殖环境控制关键技术研究与集成"(2012BAD39B02);中国农业科学院科技创新团队项目(ASTIP-IAS07)

摘　　要：本试验旨在研究不同相对湿度(RH)对间歇性26℃环境下肉鸡盲肠菌群多样性的影响。选取29日龄爱拔益加(AA)肉鸡180只转入环境控制舱,随机分成3个组(RH分别为30%、60%和85%),每组6个重复,每个重复10只鸡(公母各5只)。从29日龄开始,每天10:00—16:00(6 h)温度维持26℃,RH分别为30%、60%和85%,剩余时间温度为21℃,RH为60%。试验共14 d。采用16S rDNA的变性梯度凝胶电泳(DGGE)技术,结合特异性和共性条带割胶回收DNA进行克隆和测序,分析RH在间歇性26℃偏热处理第7天和第14天时对盲肠内容物菌群结构和多样性的影响。结果表明:1)试验第7天,30%RH组肉鸡盲肠DGGE条带数(菌群丰富程度)高于60%RH组,而85%RH组低于60%RH组;试验第14天,30%、85%RH组肉鸡盲肠DGGE条带数均高于60%RH组。2)聚类分析显示,试验第7天,85%RH对盲肠菌群影响明显;试验第14天,30%RH对盲肠菌群影响明显。但随着处理时间推移,30%RH对肉鸡盲肠菌群影响越大。3)间歇性26℃环境下不同RH组肉鸡盲肠内共性菌群是Faecalibacterium prausnitzii;在试验第7天,30%、85%RH组肉鸡盲肠中特异性菌群是Stomatobaculum longum。结果提示:间歇性26℃环境下,低湿(30%RH)和高湿(85%RH)影响肉鸡盲肠菌群的结构和多样性。This study was carried out to investigate the effects of different relative humidity （RH） on cecal mi- croflora diversity of broilers under intermittent 26 ℃ environment. One hundred and eighty 29-day-old Arbor Acres （AA） broilers were assigned to three environment chambers （RH were 30%, 60% and 85%, respec- tively）, each chamber contained six cages with ten birds per cage （ five males and five females）, and each cage as a replicate. When broilers were 29 days of age, the temperature of groups was at 26 ℃, regulating the RH to 30%, 60% and 85%, and the temperature and RH of groups were kept six hours each day at 10：00 to 16：00, and broilers were kept at 21 ℃ and 60% RH in the other time. The trial period lasted for 14 days. The effects of RH on bacterial community and diversity in the cecal digesta of broilers at the 7＇h and 14th day under intermittent 26 ℃ environment were analyzed by using 16S rDNA-based denaturing gradient gel electrophoresis （ DGGE）, combined with the cloning and sequencing of DNA recycled by specificity and generality stripe tap- ping. The results showed as follows： 1 ） on the 7th day, cecum DGGE bands number （flora abundance） of broilers in 30% RH group was higher than that in 60% RH group, while in 85% RH group was lower than that in 60% RH group; on the 14th day, cecum DGGE bands number of broilers in 30% and 85% RH groups was higher than that in 60% RH group. 2） Cluster analysis showed that on the 7th day, 85% RH affected the cecal microflora obviously, but on the 14th day, 30% RH did. And as treatment time processing, 30% RH made a greater effect on cecal microflora. 3） The common cecal microbiota of different RH groups under intermittent 26 ℃ environment was Faecalibacterium prausnitzii, and on the 7th day, the specific cecal microbiota in 30% and 85% RH groups was Stomatobaculum longum. In conclusion, at the intermittent 26 ℃ environment, 30% and 85% RH can change the structure and diversity of cecal microflora of broilers, and the eff

关 键 词：相对湿度 间歇热 肉鸡 盲肠菌群 变形梯度凝胶电泳 

分 类 号：S831.4[农业科学—畜牧学]