羌活提取物对热敏通道TRPV1的影响  

Effects of Notopterygium Incisum Extracts on the Transient Receptor Potential Vanilloid 1 of Thermosensitire Channel

作　　者：刘珍洪[1] 高琳[1] 汪文来[2] 赵红霞[2] 高蔚[1] 安致君 郭蓉[1] 吴丹桐[1] Michael Xi Zhu 杨桢[1] 

机构地区：[1]北京中医药大学,北京100029 [2]中国中医科学院中医基础理论研究所,北京100700 [3]美国德克萨斯大学休斯顿健康科学中心,休斯顿美国77030

出　　处：《中国中医基础医学杂志》2017年第4期553-557,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金面上项目(81573847)-基于热敏通道的交叉脱敏探讨理中丸;小建中汤和吴茱萸温中散寒功效的共同作用机制;北京中医药科技发展资金项目(JJ2015-56)-基于寒痛通道TRPA1的吴茱萸桂枝对宫寒小鼠的子宫容受性影响研究;北京市"3+3"李庆业名老中医工作站(2009-JC-31);中国中医科学院自主选题项目(YZ-1316)-补肾化痰散瘀法治疗多囊卵巢综合证的机理探讨

摘　　要：目的:探讨羌活水提物和醇提物对热敏通道TRPV1的影响。方法:在瞬转人源TRPV1的HEK293细胞上,运用膜片钳技术测量羌活提取物对细胞跨膜电流的影响。结果:羌活提取物可激活TRPV1通道产生明显的跨膜电流,该跨膜电流与TRPV1激动剂辣椒素诱发的电流在电流密度和电流-电压关系各方面均类似;羌活水提物在生药1 g/ml时稀释10倍左右,对跨膜电流的影响达到最大,并可以被TRPV1竞争性抑制剂辣椒素完全阻断。结论:羌活提取物中含TRPV1激动剂,该通道活化后产生的系列效应可能是羌活解表、止痛的重要分子机制。Objective： To investigate the effect of ethanol extracts and water extracts of Notopterygium incisum on thermosensitive channel, Transient Receptor Potential Vanilloid 1 （TRPVl）. Methods： Whole cell voltage clamp technique was used to record transmembrane currents induced by the Notopterygium extracts in HEK 293 cells that expressed human TRPV1. Results ： Both ethanol and water extracts of Notopterygium incisum evoked robust currents comparable to that by the known TRPV1 agonist, capsaicin, in current density and current-voltage relationship. The maximum effect of Notopterygium water extracts was reached at 100 mg/ml. The Notopterygium-induced TRPV! current was completely abolished by the specific TRPV1 antagonist, capsazepine （10 μM）. Conclusions： One or more components of Notopterygium extracts are TRPV1 agonists, which may underlie the analgesic and relieving exterior syndrome effects of Notopterygium.

关 键 词：羌活 热敏通道TRPV1 激动剂 解表 止痛 

分 类 号：R284.2[医药卫生—中药学]