miRNA在深、中低温停循环中的表达及其对肠道保护的影响  

miRNA expression between deep and moderate hypothermia circulatory arrest and its impact on intestinal protection

作　　者：林伟斌[1] 陈光献[1] 梁孟亚[1] 杨潇[1] 荣健[1] 冯康倪 秦瀚[1] 陈鉴涛 姚尖平[1] 吴钟凯[1] 

机构地区：[1]中山大学附属第一医院心脏外科,广州510000

出　　处：《中华胸心血管外科杂志》2017年第4期226-229,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金（81370215,81570039）

摘　　要：目的探讨不同温度停循环期间肠道miRNA表达是否有差异及对肠道保护的影响。方法16只猪随机分入4组：深低温停循环（DHCA，18℃）组、中低温停循环（MHCA，24℃）组、体外循环（CPB）组及假手术（s0）组。各组动物分别经历80minDHCA／MHCA、305minCPB及维持常温305min。采用定制Real—timePCR芯片检测miRNA变化情况，DHCA和MHCA对肠道miRNA的表达影响分别通过直接方法（DHCA对MHCA）和间接方法比较（DHCA／SO对MHCA／SO；DHCA／CPB对MHCA／CPB）。结果DHCA组引起肠道组织miRNA的改变数目少于MHCA组（9／304对25／304；8／304对18／304），不同温度低温停循环问7个miRNA（miR-122，miR-145—5p，miR-421-5p，miR-99a，miR-365—5p，miR-31和miR．192）表达差异有统计学意义。结论从miRNA角度而言，DHCA的肠道保护作用优于MHCA；不同温度低温停循环问肠道miRNA表达具有差异。Objective To evaluate the miRNA change between hypothermia circulatory arrest at different temperature and its impact on intestinal protection. Methods Sixteen piglets were randomly（ n =4） divided into four groups： deep hypothermia circulatory arrest （DHCA,18℃） group, moderate hypothermia circulatory arrest（ MI-ICA, 24℃ ） group, cardiopuhnonary bypass（CPB） group and sham operation（SO） group. They were subjected to 80 min hypothermia circulatory arrest, 305 min CPB or thoracotomy, respectively. Pick-and-mix custom miRNA real-time PCR panels were utilized to detect intestinal sampies. miRNA expression between DHCA and MHCA were compared directly （DHCA vs. MHCA） and indirectly （DttCA/SO vs. MHCA/SO, DHCA/CPB vs. MHCA/CPB）. Results Exposure to DHCA caused less intestinal miRNA dysregulation than MHCA. Besides, seven miRNAs （ miR-122, miR-145-5p, miR-421-Sp, miR-99a, miR-365-5p, miR-31 and miR-192） were differentially expressed between the two hypothermia circulatory arrest groups. Conclusion Better intestinal miRNA protection was provided by DHCA than MHCA. Intestinal miRNA were differentially expressed between hypothermia circulatory arrest at different temperature.

关 键 词：体外循环 模型 动物 低温 再灌注损伤 

分 类 号：R318.52[医药卫生—生物医学工程]