B-LCLs及其在单克隆抗体生产中的应用  

B-LCLs and its application in the production of monoclonal antibody

作　　者：刘玺[1,2,3] 陈新文[1] 段子渊[2] 

机构地区：[1]中国科学院武汉病毒研究所分子病毒学研究中心,武汉430071 [2]中国科学院遗传与发育生物学研究所遗传资源研究中心,北京100101 [3]中国科学院大学,北京100049

出　　处：《生命科学》2017年第4期349-355,共7页Chinese Bulletin of Life Sciences

基　　金：中国科学院重点部署项目(KFZD-SW-205)

摘　　要：B淋巴母细胞系(B-LCLs)体外可以无限增殖,为很多实验持续提供基因型和表型匹配的原材料,极大地促进了生物医学研究的发展。近年来,B-LCLs在单克隆抗体的生产、人类全基因组分析等方面得到重要应用,成为遗传进化研究的重要资源。现对B淋巴母细胞系及其在单克隆抗体生产中的应用作一简述。B lymphoblastoid cell lines （B-LCLs）, which can proliferate indefinitely in vitro, fulfil the requirement of constant supply of starting material with matching genotypes and phenotypes for variety of assays, substantially accelerating the process of biomedical investigations. In recent years, its utility for producing monoclonal antibody and analyzing the whole human genome has extensively been documented. B-LCLs are increasingly being considered as an important resource for genetic and fimctional research. This article reviews the B-LCLs and its application in the production of monoclonal antibody.

关 键 词：EB病毒 B淋巴母细胞系 单克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]