白藜芦醇对高原脑水肿小鼠血管内皮生长因子表达的影响  

Effect of resveratrol on the expression of VEGF in mice with high-altitude cerebral edema

作　　者：王敏[1] 孙世中[2] 罗玉玉[3] 李海英[3] 赵娟[3] 张璐[4] 

机构地区：[1]天津中医药大学,天津300193 [2]武警后勤学院附属医院神经重症科,天津300162 [3]武警后勤学院临床医学系生理学与病理生理学教研室,天津300309 [4]武警后勤学院科研部,天津300309

出　　处：《武警后勤学院学报（医学版）》2017年第1期25-27,36,F0003,共5页Journal of Logistics University of PAP（Medical Sciences）

基　　金：国家自然科学基金资助项目(81370439);武警后勤学院面上项目(WHM201207);武警后勤学院博士启动金项目(WHB201302)

摘　　要：【目的】研究白藜芦醇对高原脑水肿小鼠脑组织的保护作用及血管内皮生长因子(vascular endothelial growth factor,VEGF)基因及蛋白表达的影响。【方法】成年雄性昆明种小鼠随机分为5组:常氧对照组、急性低氧组、白藜芦醇大、中、小剂量组;不同浓度白藜芦醇预处理小鼠1周,将其放入低压氧舱,模拟海拔8 000 m高度,缺氧24 h。HE染色观察小鼠脑组织形态学改变,real-time PCR检测VEGF mRNA表达变化,免疫组化和Western blotting方法检测VEGF蛋白表达变化。【结果】与急性低氧组比较,白藜芦醇预处理组小鼠脑组织形态结构接近常氧对照组,VEGF mRNA及蛋白表达均明显下调(P<0.05)。【结论】白藜芦醇能够减轻急性低压低氧导致的脑水肿,保护脑组织,此作用与其下调VEGF的表达,保护血脑屏障通透性有关。[Objective] To study the protective effect of resveratrol on brain tissues and its regulation on the expression of vascular endothelial growth factor （VEGF） in mice with high-altitude cerebral edema. [Methods] The male Kunming mice were randomly divided into 5 groups： control group, acute hypoxia group, intervention groups with resveratrol in high, ＂medium and low dose. The mice were pretreated with different concentrations of resveratrol for 1 w and placed in a hypobaric chamber which mimicked the height of 8 000 m above sea level for 24 h. The microstructure changes in the cerebral tissues of mice were observed via HE staining, real-time PCR, immunohistochemistry and Western blotting were used for detecting the mRNA and protein expressions of VEGF. [Results] Compared with acute hypoxia group, the microstructures were improved in the intervention groups with resveratrol. The mRNA and protein levels of VEGF were down-regulated obviously （P〈 0.05）. [ Conclusion ] ResveratroI can attenuate cerebral edema caused by hypobarie hypoxia and protect brain from injury. This action may be related with its effect of down- regulating the expression of VEGF.

关 键 词：白藜芦醇 高原脑水肿 血管内皮生长因子 

分 类 号：R965[医药卫生—药理学]