2株海鞘来源放线菌Streptomyces coelicoflavus和Nocardiopsis dassonvillei的次级代谢产物及其生物活性研究  

Secondary metabolites and their biological activities of two actinomycetes Streptomyces coelicoflavus and Nocardiopsis dassonvillei associated with ascidians Styela clava and Botryllus schlosseri

作　　者：裴召苓 陈雷 许嘉磊 邵长伦[2] 

机构地区：[1]哈尔滨工业大学(威海)海洋科学与技术学院,山东威海264209 [2]中国海洋大学海洋药物教育部重点实验室医药学院,山东青岛266003

出　　处：《中国海洋药物》2017年第2期55-60,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(31300009);国家海洋局海洋公益性行业科研专项(201405038);山东省自然科学基金(JQ201510);哈尔滨工业大学科研创新基金(HIT.NSRIF.2014127);哈尔滨工业大学威海校区学科建设引导基金(WH20150204和WH20160205)资助

摘　　要：目的从采自中国黄海的威海海域的柄海鞘来源放线菌Streptomyces coelicoflavus(HQA809)和史氏菊海鞘来源放线菌Nocardiopsis dassonvillei(HQA404)中分离、鉴定具有生物活性的次级代谢产物。方法运用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备HPLC等方法分离化合物,运用核磁、质谱等现代波谱分析方法对化合物进行结构鉴定,并对化合物进行抗菌、卤虫致死、α-糖苷酶抑制活性和细胞毒活性评价。结果从Streptomyces coelicoflavus(HQA809)中分离鉴定了2个吡喃酮类化合物(1,2),从Nocardiopsis dassonvillei(HQA404)中分离鉴定了1个吩嗪类化合物(3)和1个苯酚类化合物(4);化合物3对鳗弧菌(Vibrio anguillarum)和副溶血弧菌(Vibrio parahaemolyticus)显示出较强的抑菌活性,其MIC值分别为50μmol/L和25μmol/L。结论海鞘来源放线菌可以作为发现新颖的生物活性物质的潜在来源。Objective To isolate bioactive natural compounds from the two actinomycete strains Strepto- myces coelico flavus （HQAS09 ） and Nocardiopsis dassonvillei （HQA404） associated with the ascidians Styela clara and Botryllus schlosseri collected from the coast of Weihai in the Yellow Sea of China. Methods The compounds isolated from the actinomycete strains Streptornyces coelicoflavus （HQA809） and Nocardiopsis dassonvillei （HQA404） were separated and purified by silica gel column chromatography, Sephadex LH-20 gel and HPLC. The structures of these isolated compounds were identified by modern spectroscopic analysis including NMR and MS methods. In addition, the biological activities of the isolated compounds were evaluated for the antibacterial activity, brine shrimp lethality, α-glucosi- dase inhibitory activity and cytotoxic activity. Results Two pyrones （1, 2） were isolated from HQA809, one phenazine compound （3） and one phenol derivative （4） were isolated from HQA404. Compounds 3 showed antibacterial activities against Vibrio anguillarum and Vibrio parahaemolyticus with MIC values of 50μmol/L and 25 μmol/L, respectively. Conclusion The results suggested that actinomycetes associated with ascidians might be developed as promising sources for the discovery of novel bioactive substances.

关 键 词：海鞘 放线菌 化合物 生物活性 

分 类 号：R931[医药卫生—生药学]