噬菌体遗传改造技术研究进展  

Progress on bacteriophage genetic engineering

作　　者：谢晓渝 乐率[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所急诊科,重庆400038 [2]第三军医大学基础部微生物学教研室,重庆400038

出　　处：《生命的化学》2017年第2期171-175,共5页Chemistry of Life

基　　金：国家自然科学基金项目(31501004)

摘　　要：噬菌体在20世纪初被发现时,其抗感染的潜力就受到极大重视。但随着抗生素的问世,噬菌体治疗被逐渐忽略。近年来,多重耐药菌的不断出现和新型抗生素的缺乏使得噬菌体治疗再次受到全球关注。对噬菌体进行精确的遗传改造可以使之更好的发挥抗感染作用。此外,噬菌体通过遗传改造还可以用于药物传递、疫苗展示、病原体诊断等众多生物医药领域,具有极大的应用前景。本文着重对噬菌体遗传改造技术及其应用进展进行综述。When bacteriophage was first discovered in the early twentieth Century, its antimicrobial poten- tial was very attractive. But, with the discovery of antibiotics, phage therapy is gradually ignored. In recent years, the emergence of multi-drug resistant bacteria and the lack of new antibiotics make phage therapy a new option to cure bacterial infection. The precise genetic modification of the phage will significantly improve its antimicrobial efficiency. In addition, the genetically engineered phages can also be used in the delivery of drug, vaccine display, pathogen diagnosis and other pharmaceutical fields. Therefore, this review focuses on the progress of phage genetic modification technology and its applications in recent years.

关 键 词：噬菌体 遗传改造 噬菌体治疗 

分 类 号：Q933[生物学—微生物学] R978[医药卫生—药品]