半夏泻心汤对胃癌前病变大鼠胃黏膜组织中的NF-κB/STAT3信号通路的影响研究  

Effect of Banxia Xiexin decoction on NF-κB/STAT3 in gastric mucosa of PLGC rats

作　　者：李慧臻[1] 刘琳[1] 王兴章[2] 刘华一 杨岩[1] 赵双梅[1] 张淑坤 李棣华[4] 

机构地区：[1]天津中医药大学第二附属医院脾胃病科,天津300150 [2]天津中医药大学研究生院,天津300193 [3]天津市中医研究院附属医院脾胃病科,天津300120 [4]天津市中西医结合医院南开医院急腹症研究所,天津300100

出　　处：《中国中西医结合消化杂志》2017年第4期284-288,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然基金资助项目(No:81273708)

摘　　要：[目的]通过检测大鼠胃黏膜组织中的NF-κB/STAT3信号通路中等相关指标,揭示半夏泻心汤对胃癌前病变(PLGC)大鼠的影响及防治作用机制。[方法]130只清洁级雄性SD大鼠,采用改良MNNG+复合法造模PLGC大鼠110只,分为模型组:PLGC大鼠20只;模型中药组:造模同时中药干预10只;空白组:正常喂养20只。中药干预阶段,剩余模型大鼠随机分为模型对照组10只、半夏泻心汤高、中、低剂量组分别为12、12、10只。观察检测各组大鼠胃黏膜组织中的NF-κB、STAT3、IL-1β、TNF-α、Bcl-2、C-MYC、p21情况。[结果]中药干预后,NF-κB:模型中药组比模型组表达低,高剂量组、中剂量组及低剂量组均比模型对照组表达低,高剂量组、中剂量组明显;STAT3:模型中药组比模型组表达低,高剂量组、中剂量组及低剂量组均比模型对照组表达低,高剂量组、中剂量组、低剂量组间差异无统计学意义;IL-1β:模型中药组比模型组表达低,高剂量组、中剂量组及低剂量组均比模型对照组表达低,中剂量组明显。TNF-α:模型中药组比模型组表达低,高剂量组、中剂量组及低剂量组均比模型对照组表达低,低剂量组明显。Bcl-2:模型中药组比模型组表达低,高剂量组、中剂量组比模型对照组表达低,高剂量组明显;C-MYC:模型中药组比模型组表达低,高剂量组、中剂量组比模型对照组表达低,高剂量组明显;p21:模型中药组比模型组表达高,高剂量组、中剂量组比模型对照组表达高,高剂量组明显。[结论]中药半夏泻心汤通过抑制PLGC大鼠胃黏膜组织NF-κB/STAT3信号通路中的炎性因子、癌因子,促进抑癌因子的表达,从而影响阻断PLGC的发生发展。[Objective]To explore the effect of Banxia Xiexin decoction on NF-κB/STAT3 in gastric mucosa of PLGC rats and to investigate the potential function. [Methods]Modified MNNG -- complex method was used to establish PLGC rat model in 110 rats, which were then divided into 2 groups at random, model group, and Chinese medicine group. Another 20 rats were taken as normal control. At the stage of Chinese medicine intervention, the remaining model rats were randomly divided into 4 groups. 10 rats in model control group, 12, 12 and 10 rats were in the high, medium and low dose group. The level of NF- κB, STAT3, Bcl-2, C-MYC, and p21 in the gastric mucosa of rats in each group were observed. [Results] The expression of NF-κB, STAT3, Bcl-2, and C-MYC in Chinese medicine intervention groups was signif- icantly lower than that in model group, while p21 showed opposite result, much higher in Chinese medicine group than model group. [Conelusion]Banxia Xiexin decoction can inhibit the inflammatory factors and cancer factors in the NF κB/STAT3 signaling pathway in the gastric mucosa of PLGC rats.

关 键 词：胃癌前病变 半夏泻心汤 NF-ΚB STAT3 

分 类 号：R285.5[医药卫生—中药学]