人小涎腺间充质干细胞与骨髓间充质干细胞的增殖和成脂比较  

Comparative Study on Proliferative and Adipogenic Ability of Human Minor Salivary Gland Mesenchymal Stem Cells and Bone Marrow Mesenchymal Stem Cells

作　　者：曹蕊 严笠 张辰 孙雪健 赵振民 

机构地区：[1]中国医学科学院整形外科医院研究中心,北京100144

出　　处：《中国美容医学》2017年第5期31-35,共5页Chinese Journal of Aesthetic Medicine

摘　　要：目的:通过对人小涎腺干细胞(human Minor Salivary Gland Mesenchymal Stem Cells,hMSGMSCs)与人骨髓间充质干细胞(human Bone Marrow Mesenchymal Stem Cells,hBMSCs)的增殖、活力、凋亡以及成脂分化能力等生物学特性的比较,证实小涎腺干细胞具有高度自我更新能力并具有向脂肪细胞分化的潜能,为其成为新型的理想种子细胞提供理论基础。方法:提取临床患者的hMSGMSCs和hBMSCs各3例,利用MTS细胞增殖与毒性检测试剂盒,检测两种细胞的490nm吸光度值(optical density,OD490nm)的变化并绘制增殖曲线,比较两种细胞的增殖能力;利用MUSE细胞分析仪检测两种细胞的活力和凋亡,并加以比较;对两种细胞进行成脂诱导,2周后油红O染色,镜下观察及用异丙醇溶解成脂细胞吸附的油红O染料,检测OD490nm,比较脂滴形成情况。结果:hMSGMSCs比hBMSCs具有更强的增殖能力(P<0.05),将其传至30代仍很好地保持细胞的活性;两种干细胞活力及凋亡情况检测无明显差异(P>0.05);两种细胞成脂诱导2周后OD490nm无明显差异(P>0.05)。结论:hMSGMSCs将有望成为理想的种子细胞,应用于细胞治疗和再生医学研究。Objective To identify the self-renewal and adipogenic differentiation potential and explore ideal seed cells for cell therapy and bioengineered organ, we compared the biological characters including cell proliferation,vitality, apoptosis and adipogenic differentiation ability between human minor salivary gland mesenchymal stem cells （hMSGMSCs） and human bone marrow mesenchymal stem cells （hBMSCs）. Methods hMSGMSCs and hBMSCs derived from 3 patients were isolated and cultured. 3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sul-fophenyl）-2H-tetrazolium, inner salt （MTS） assay were used to detect proliferative ability of cells, and the growth curve was plotted according to the absorbance of 490 nm. Cell vitality and apoptosis were measured by MUSE cell analyzer. The adipogenic differentiation of cells was induced for two weeks, and the lipid droplets formation in cells were stained with the Oil Red O and were observed under the microscope, and the optical density at 490 nm （OD490nm） was detected after being dissolved in isopropanol. Results The proliferative ability of hMSGMSCs was higher than hBMSC, and hMSGMSCs could maintain stem cell vitality until the passage 30 （P〈0.05）. No statistically significant differences of the cell vitality, apoptosis and adipogenic differentiation ability between hMSGMSCs and hBMSCs were found （P〉0.05）. Conclusion hMSGMSCs has the potential to become one kind of desirable seed cell for basic research and cell therapy in regenerative medicine.

关 键 词：小涎腺干细胞 骨髓间充质干细胞 细胞增殖曲线 凋亡 成脂诱导 

分 类 号：Q813.1[生物学—生物工程]