两种示踪技术在人脐带间充质干细胞治疗子痫前期大鼠模型中的应用  

Application of two kinds of tracer techniques for human umbilical cord mesenchymal stem cells in treatment of preeclampsia rats

作　　者：王蕾蕾[1] 付丽华[2] 张丹[3] 高漫[1] 刘彤[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]首都医科大学附属北京地坛医院妇产科,北京100011 [3]沈阳市妇婴医院妇产科,辽宁沈阳110014

出　　处：《中国妇幼保健》2017年第10期2215-2220,共6页Maternal and Child Health Care of China

摘　　要：目的探讨含绿色荧光蛋白慢病毒(LV-GFP)和荧光染料四甲基-吲哚羧花青-高氯酸盐(CM-Di I)两种标记干细胞的示踪技术在人脐带间充质干细胞(HU-MSCs)治疗子痫前期(PE)大鼠研究中的应用。方法采用酶消化法分离培养HU-MSCs,分别以LV-GFP和CM-Di I标记HU-MSCs。建立脂多糖(LPS)诱导的PE大鼠模型,移植HU-MSCs后分别通过荧光显微镜和活体成像技术示踪HU-MSCs的分布。结果荧光显微镜下见LV-GFP标记的HU-MSCs分布于大鼠胎盘组织和肾脏组织中;胎鼠肾脏、肝脏、心脏和肺脏组织中未见分布。活体成像技术显示CM-Di I标记的HU-MSCs分布于大鼠胸腹部脏器及胎盘,而脐带及胎鼠内未见分布。结论 LV-GFP标记后荧光显微镜可显示最终HU-MSCs归巢并定位准确,CM-Di I标记后活体成像技术则可以观察活体内HU-MSCs分布变化。Objective To explore the application of LV-GFP tracer technique and CM-DiI tracer technique for human umbilical cord mesenchyma! stem cells （U-MSCs） in treatment of rats with preeclampsia. Methods HU-MSCs were isolated and cultured by enzymatie digestion method, model was established, then HU-MSCs were labeled by LV-GFP and CM-DiI, respectively. Lipopolysaccharide-induced preeclampsia rat after transplant of HU-MSCs, fluorescence microscope and bioluminescence imaging technique were used to track the distributions of HU-MSCs, respectively. Results HU-MSCs were found in placenta and kidney of preeclampsia rats, but HU-MSCs were not found in kidney, liver, heart, and lung of fetal rats. HU-MSCs labeled by CM-DiI were found in thoracic and abdominal organs as well as placenta, and HU-MSCs were not found in umbilical cord and fetal rats. Conclusion LV-GFP-labeled HU-MSCs location can be tracked ＇acburately under fluorescence microscope, the change of CM-DiI-labeled HU-MSCs distribution can be observed by bioluminescence imaging technique.

关 键 词：子痫前期 人脐带间充质干细胞 细胞示踪 

分 类 号：R714[医药卫生—妇产科学]