GMDTC驱镉剂量研究  

Study on the dosage of GMDTC for removing cadmium

作　　者：曹丽芳[1,2] 钟志勇[2] 唐伟[2] 董明[3] 付四海[2] 仇玉兰[1] 任雪峰[2] 唐小江[2] 

机构地区：[1]山西医科大学公共卫生学院,山西太原030001 [2]广东省医学实验动物中心,广东佛山528248 [3]广东省职业病防治院广东省职业病防治重点实验室,广州510300

出　　处：《中国职业医学》2017年第2期142-147,共6页China Occupational Medicine

基　　金：广东省科技计划项目(2016A030310319);广东省科技计划重大专项(2012A080201011);国家重大新药创制科技重大专项入库课题(2015GKH-384);佛山市科技计划项目(2012HY100302)

摘　　要：目的探讨静脉滴注驱镉剂N-(2,3,4,5,6-五羟基己基)-(N-二取代甲酸钠基)-L-甲硫氨酸钠(GMDTC)不同剂量下的驱镉效果。方法雄性新西兰兔随机分为空白对照组、GMDTC高剂量对照组、模型对照组、依地酸钠钙(EDTA)对照组和GMDTC低、中、高剂量组,每组5只。空白对照组和GMDTC高剂量对照组新西兰兔分别经耳缘静脉予0.90%氯化钠注射液,模型对照组、EDTA对照组和3个GMDTC剂量组新西兰兔分别经耳缘静脉予浓度为2μmol/kg氯化镉及浓度为40μmol/kg的β-巯基乙醇的混合溶液,给药量为5.0 mL/kg体质量,1次/d,连续5 d。于造模第41天即治疗第1天,空白对照组和模型对照组新西兰兔均经耳缘静脉予0.90%氯化钠注射液250 mL,EDTA对照组新西兰兔予剂量为93.5 mg/kg体质量的EDTA溶液,GMDTC高剂量对照组和3个GMDTC剂量组新西兰兔分别予剂量为108.0、12.0、36.0、108.0 mg/kg体质量的GMDTC溶液,1次/d,6次/周,连续4周。检测各组新西兰兔治疗前后尿β_2-微球蛋白(β_2-MG)、肾镉、血镉和尿镉水平。结果实验期间,新西兰兔体质量随饲养时间的增加而增加(P<0.01)。模型对照组、EDTA对照组和3个GMDTC剂量组新西兰兔治疗前尿β_2-MG水平均高于空白对照组(P<0.05)。GMDTC中、高剂量组新西兰兔治疗后肾镉水平均低于模型对照组和EDTA对照组(P<0.05)。EDTA对照组和3个GMDTC剂量组新西兰兔治疗后的血镉水平均低于同组治疗前(P<0.05),且均低于治疗后的模型对照组(P<0.05);GMDTC中、高剂量组和EDTA对照组新西兰兔治疗后血镉水平两两比较,差异均无统计学意义(P>0.05)。治疗后各时间点(治疗后第1、6、8、13、15、20、22和28天)的新西兰兔尿镉水平,在EDTA对照组和3个GMDTC剂量组均低于同时间点模型对照组(P<0.05);治疗后除第20和22天外,其余6个时间点新西兰兔尿镉水平均随GMDTC剂量的增加而增加(P<0.05)。GMDTC低、中、高剂量组新西兰兔治疗后血镉Objective To explore different doses of sodium（s）-2-（dithiocarboxylato（（2R,3R,4R,5R,6R）-2,3,4,5,6-pentahydroxyhexyl） amino）-4-（methylthio） butanoate （GMDTC） for removing cadmium.Methods Thirty-five male New Zealand rabbits were randomly divided into blank control group,GMDTC high dose control group,model control group,ethylene diamine tetraacetic acid （EDTA） control group and GMDTC low,medium and high dose groups,five rabbits in each group.The blank control group and GMDTC high dose control group were given 0.90% normal saline solution intravenously;model control group,EDTA control group and GMDTC low,medium and high dose group were given 2 μmol/kg of cadmium chloride （CdCl2） and 40 μmol/kg of β-mercaptoethanol mixed solution intravenously,5.0 mL/kg body weight （bw）,once a day for five days.On the forty-one day of the experiment （the fist day of GMDTC treatment）,the control group and the model control group were injected 0.90% normal saline solution 250 mL via ear vein,the EDTA control group was given EDTA solution at the dose of 93.5 mg/kg bw with 250 mL 0.90% normal saline solution,also via ear vein;the GMDTC high dose control group,and the GMDTC low,medium and high dose groups were given 250 mL GMDTC solution at the concentration of 108.0,12.0,36.0 and 108.0 mg/kg bw with 0.90% normal saline by intravenous infusion,once a day,6 times a week for four consecutive weeks.The urine β2-microglobulin （MG）,renal cadmium,blood cadmium,and urinary cadmium before and after the treatment were detected.Results The body weight of New Zealand rabbits increased with the increasing feed time （P〈0.01）.The levels of β2-MG before treatment increased in model control group,EDTA control group,GMDTC low,medium and high dose groups than that in the blank control group （P〈0.01）.The levels of renal cadmium after treatment in GMDTC medium and high dose groups decreased compared with those in the blank control group and EDTA control group respectively （P〈0.05）.The blood c

关 键 词：N-(2 3 4 5 6-五羟基己基)-(N-二取代甲酸钠基)-L-甲硫氨酸钠 镉中毒 尿镉 血镉 肾镉 新西兰兔 

分 类 号：R135.1[医药卫生—劳动卫生]