金银花炒炭前后6种成分的含量变化研究  

Study on Content Changes of 6 Components in Lonicera japonica before and after Carbonized

作　　者：吴明侠[1] 李会[1] 崔永霞[1] 侯珊珊[1] 丁亚慧[1] 

机构地区：[1]河南中医药大学分析测试中心,郑州450046

出　　处：《中国药房》2017年第15期2112-2114,共3页China Pharmacy

基　　金：河南省高等学校重点科研项目(No.17A360022);河南中医药大学科研苗圃工程项目(No.MP2016-17)

摘　　要：目的:建立同时测定金银花中6种成分含量的方法,并研究金银花炒炭前后上述成分含量的变化。方法:采用超高效液相色谱法。色谱柱为Agilent Eclipse Plus C_(18)RRHD,流动相为0.1%磷酸溶液-乙腈(梯度洗脱),流速为0.2 mL/min,检测波长为350 nm,柱温为25℃,进样量为1μL。结果:绿原酸、芦丁、木犀草苷、异绿原酸A、异绿原酸B、异绿原酸C检测进样量线性范围分别为21.2~424μg(r=0.999 3)、1.17~23μg(r=0.999 5)、2.18~43μg(r=0.999 8)、5.10~102μg(r=0.999 3)、2.60~52μg(r=0.999 1)、4.95~99μg(r=0.999 8);精密性、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.11%~99.76%(RSD=1.20%,n=6)、95.20%~99.90%(RSD=2.20%,n=6)、95.71%~100.30%(RSD=2.20%,n=6)、95.00%~96.98%(RSD=0.88%,n=6)、96.47%~103.00%(RSD=2.40%,n=6)、95.78%~103.80%(RSD=3.20%,n=6)。与炮制前比较,炮制后的金银花中芦丁、异绿原酸B、异绿原酸C的含量增加,绿原酸、异绿原酸A的含量明显下降,而木犀草苷的含量无明显变化。结论:该方法操作简便,精密度、稳定性、重复性好,可用于金银花中6种成分含量的同时测定;金银花炒炭前后化学成分有一定变化。OBJECTIVE： To establish the method for simultaneous determination of 6 components in Lonicera japonica, and to study the content changes of them before and after before and after carbonized. METHODS： UPLC method was adopted. The determination was performed on Agilent Eclipse Plus C18 RRHD column with mobile phase consisting of 0.1% phosphoric acid solution-acetonitrile （gradient elution） at the flow rate of 0.2 mL/rain. The detection wavelength was set at 350 nm, and column tem- perature was 25℃. The sample size was 1 μL. RESULTS： The linear ranges of ehlorogenic acid, rutin, galuteolin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C were 21.2-424μg（r=0.999 3）, 1.17-23 μg（r=0.999 5）, 2.18-43 μg（r= 0.999 8）, 5.10-102 μg（r=0.999 3）, 2.60-52 μg（r=0.999 1）, 4.95-99μg（r=0.999 8）, respectively. RSDs of precision, stability and repeatability tests were all lower than 2.0%. Recoveries were 97.11%-99.76% （RSD=1.20% ,n=6）, 95.20%-99.90% （RSD=2.20%,n=6）, 95.71%-100.30%（RSD=2.20%,n=6）, 95.00%-96.98%（RSD=0.88%,n=6）, 96.47%-103.00%（RSD=2.40%, n=6）, 95.78%-103.80% （RSD=3.20%, n=6）. Compared with before processing, the contents of rutin, isochlorogenic acid B and isochlorogenic acid C in L. japonica were increased along with processing, the contents of chlorogenic acid and isochlorogenic acid A were decreased significantly, while the content of galuteolin had no significant change. CONCLUSIONS： The method is sim- ple, precise, stable and repeatable, and can be used for simultaneous determination of 6 components in L. japonica. Those chemi- cal components have certain changes before and after carbonized.

关 键 词：金银花 炒炭 超高效液相色谱法 含量 变化 

分 类 号：R927.2[医药卫生—药学]