瑞香狼毒对东亚飞蝗作用机理的初步研究  

Studies on the mechanism of Stellera chamaejasme L.to Locusta migratoria manilensis(Meyen)

作　　者：陈琼[1] 高甜甜[1] 李波[1] 查艳梅 陶科[1] 金洪[1] 侯太平[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2017年第3期665-669,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31272068);四川省应用基础研究项目(2014JY0063)

摘　　要：利用硅胶柱分离瑞香狼毒乙酸乙酯萃取物得到Ⅰ、Ⅱ、Ⅲ段粗分物,并测定对东亚飞蝗毒杀活性最高的I段粗分物对东亚飞蝗的致死性及对其体内Na^+-K^+-ATPase、Ca^(2+)/Mg^(2+)-ATPase、乙酰胆碱酯酶的抑制活性.研究表明:瑞香狼毒I段粗分物对东亚飞蝗具有较强的毒杀作用,在药物浓度为10mg/mL、72h对东亚飞蝗的校正死亡率为92.50%;同时,毒杀作用具有浓度和时间依赖性;而且瑞香狼毒乙酸乙酯萃取物I段粗分物对上述3种酶均有一定的抑制作用,且对Na^+-K^+-ATPase的抑制作用最强,在药物浓度为5mg/mL、96h对Na^+-K^+-ATPase的抑制率为40.38%;分析发现瑞香狼毒活性成分对东亚飞蝗的毒杀作用与对Na^+-K^+-ATPase的抑制作用之间具有显著相关性,据此推测,Na^+-K^+-ATPase可能是瑞香狼毒活性成分的主要作用靶标之一.Using silica gel column to separate the ethyl acetate extract of Stellera chamaejasme L. we can get Ⅰ,Ⅱ,Ⅲ sections. The activities of Na＋-K＋-ATPase,Ca2＋/Mg2＋-ATPase and acetylcholinesterase of insects were determined in vivo. The results showed that I section from S. chamaejasrne L. had the strongest poison effect on Locusta migratoria manilensis （Meyen）. Under the concentration of 10 mg/ mL, the corrected mortality was 92.50% in 72 hour. And the poison effect there was persistence. The I section from S. chamaejasme L. also had certain inhibition on activity of Na＋-K＋-ATPase,Ca2＋/Mg2＋- ATPase,acetylcholinesterase. The inhibiton rate of Na＋-K＋-ATPase was the highest among the three kinds of enzymes. There was a well correlation between the toxic action of the active ingredients from S. chamaejasme L. to L. migratoria manilensis （Meyen） and the inhibition on activity of Na＋-K＋-ATPase in L. migratoria manilensis （Meyen）. Na＋-K＋-ATPase is likely the target of the active ingredients from S. chamaejasme L. against L. migratoria manilensis （Meyen）.

关 键 词：瑞香狼毒 东亚飞蝗 Na+-K+-ATPase Ca2+/Mg2+-ATPase 乙酰胆碱酯酶 

分 类 号：S43[农业科学—农业昆虫与害虫防治]