大枣叶中甜味抑制剂的提取与纯化工艺  

The extraction and purification process of sweetness inhibitor from Ziziphus jujuba leaves

作　　者：刘世军[1,2,3] 王欢欢[1,2,3] 唐志书[1,2,3] 崔春利[1,2,3] 刘红波[1,2,3] 梁艳妮[1,2,3] 张娱[1,2,3] 

机构地区：[1]陕西中医药大学/陕西省中药资源产业化协同创新中心,咸阳712083 [2]陕西省中药基础与新药研究重点实验室,咸阳712083 [3]陕西省风湿与肿瘤类中药制剂工程技术研究中心,咸阳712083

出　　处：《中国食品添加剂》2017年第3期74-79,共6页China Food Additives

基　　金：陕西省科技统筹创新工程计划项目(2015KTCL03-14);陕西省教育厅服务地方专项计划项目(15JF001);陕西省科技资源开放共享平台项目(2015FWPT-01);中药产业过程大学生创新实践基地项目[陕教财(2013)171号]

摘　　要：目的优化大枣叶中甜味抑制剂的提取及纯化工艺。方法采用L_9(3~4)正交设计法安排实验,以浸膏得率,甜味抑制剂提取率为考察指标,筛选最佳提取方法,再用不同型号大孔树脂吸附法纯化大枣叶中的甜味抑制剂。结果优选出最佳水提工艺为加入8倍量的水,浸泡30min,提取3次,每次1小时。最佳纯化工艺为用HPD-600型大孔吸附树脂吸附,70%乙醇洗脱,纯度最高。结论优选的提取及纯化方法稳定、合理,可为大生产提供依据。Objective ： The objective of this study was to optimize the process of sweet-inhibitors and investigate the purification conditions of macroporous resin for sweet-inhibitors from jujube leaves. Methods The process was optimized by L9 （3^4） orthogonal design, and purifying methods was selected by different macroporous resin adsorption method. Results ： The optimum extracting condition was soaking in water for half an hour, extraction 1 hours, 3 times, the amount of water used was 8 times of the leaves weight. The highest purification rate was obtained by HPD-600 macroporous adsorption resin, and 70% purification method is stable, feasible, reasonable and reqinhla ethanol elufion. Conclusion ： The process and optimal

关 键 词：大枣叶 正交试验 大孔吸附树脂 甜味抑制剂 纯化 

分 类 号：TS202.1[轻工技术与工程—食品科学]