补体C3a受体拮抗剂对三氯乙烯致敏小鼠肾脏免疫性损伤的影响  

The effect of complement C3a receptor antagonist in the kidney immune injury in trichloroethylene-sensitized mice

作　　者：杨鹏[1] 臧丹丹[1] 杨小东[1] 李博栋 王贤[1] 张家祥[1] 朱启星[2] 

机构地区：[1]安徽医科大学公共卫生学院职业卫生与环境卫生学系,合肥230032 [2]安徽医科大学皮肤病研究所

出　　处：《中华劳动卫生职业病杂志》2017年第3期161-165,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：基金项目：国家自然科学基金（81371730、81673141、81502791）;高等学校博士学科点专项科研基金（20133420110001）

摘　　要：目的 通过使用C3a受体特异性拮抗剂（C3aRA）研究补体C3a-C3aR在三氯乙烯（TCE）致敏小鼠肾脏免疫性损伤中的作用,探讨职业性三氯乙烯药疹样皮炎（ODMLT）肾脏损伤机制.方法 将42只无特定病原体（SPF）级雌性BALB/c小鼠（6～8周）随机分为空白对照组（5只）、溶剂对照组（5只）、TCE处理组（16只）和TCE＋C3aRA处理组（16只）,其中TCE处理组与TCE＋C3aRA处理组根据皮肤致敏试验评分进一步区分为致敏组和非致敏组.取小鼠血清样品采用生化试剂盒检测小鼠肾功能变化;采用实时荧光定量多聚核苷酸链式反应（qPCR）法检测小鼠肾脏组织中C3aR基因表达水平变化;采用免疫组化法检测小鼠肾脏组织中白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）蛋白表达水平变化.结果 与溶剂对照组和相应非致敏组比较,TCE致敏组和TCE＋C3aRA致敏组小鼠肌酐（CRE）和尿素氮（BUN）水平明显升高,差异均有统计学意义（P＜0.05）;与TCE致敏组比较,TCE＋C3aRA致敏组CRE和BUN水平明显降低,差异均有统计学意义（P＜0.05）.与溶剂对照组和TCE非致敏组比较,TCE致敏组小鼠肾脏组织中C3aR基因表达水平均明显升高,差异均有统计学意义（P＜0.05）.TCE致敏组和TCE＋C3aRA致敏组小鼠肾脏组织中IL-1β和TNF-α蛋白有大量表达,与TCE致敏组比较,TCE＋C3aRA致敏组肾脏组织中IL-1β和TNF-α蛋白表达水平明显降低,差异均有统计学意义（P＜0.05）.结论 补体C3a-C3aR可能参与TCE致敏小鼠肾脏免疫性损伤,C3aRA对TCE致敏小鼠肾脏免疫性损伤有一定的保护作用.Objective To explore the effect of complement C3a-C3a receptor in the kidney immune injury in trichloroethylene-sensitized mice by using C3a receptor specific antagonist C3aRA and discuss the pathogenesis of kidney injury in occupational dermatitis medicamentosa-like of trichloroethylene （ODMLT）.Methods 42 female 6～8 weeks old BALB/c mice of specific pathogen free were randomly divided into blank control group （5）,solvent control group （5）,TCE treatment group （16） and TCE＋C3aRA treatment group （16）.The TCE treatment group and TCE ＋C3aRA treatment group were further divided into the sensitized group and the non-sensitized group according to the skin sensitization test score.Renal function was detected by biochemical detection kit;expression of C3aR in kidney tissue was detected by qPCR;expression of IL-1β and TNF-α protein were detected by immunohistochemical.Results Compared with solvent control group and corresponding non-sensitized group,CRE and BUN in TCE sensitized group and TCE＋C3aRA sensitized group were significantly increased （P〈0.05）.Compared with TCE sensitized group,CRE and BUN in TCE ＋C3aRA sensitized group were significantly decreased （P〈0.05）.Compared with solvent control group and TCE non-sensitized group,the expression level of C3aR gene in kidney tissue in TCE sensitized group was significantly increased （P〈0.05）.There was a large number of IL-1β and TNF-α protein expression in kidney tissue in TCE sensitized group and TCE ＋C3aRA sensitized group.Compared with the TCE sensitized group,thc cxpression level of IL-1β and TNF-α protein in kidney tissue in TCE＋C3aRA sensitized group was significantly decreased（P〈0.05）.Conclusion C3a-C3aR maybe involved in the kidney immune injury in TCE sensitized mice,C3aRA has a protective effect on the kidney immune injury in TCE sensitized mice.

关 键 词：三氯乙烯 肾 C3a受体 白细胞介素类 肿瘤坏死因子 

分 类 号：R135[医药卫生—劳动卫生]