彗星尾部DNA含量对苯接触工人血细胞数量变化的预测  

Value of DNA content in comet tail in predicting changes in blood cell counts in workers exposed to benzene

作　　者：梁博萱 刘学星[1] 陈丽萍[1] 杨爱初[2] 黄明[2] 邓棋霏[1] 陈雯[1] 肖勇梅[1] 

机构地区：[1]中山大学公共卫生学院,广州市环境污染与健康风险评价重点实验室,广州510080 [2] 广东省职业病防治院

出　　处：《中华劳动卫生职业病杂志》2017年第3期171-175,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：基金项目：国家自然科学基金（81372962、81430079、81402658）;十二五国家科技支撑计划课题（2014BA112801）

摘　　要：目的 探讨彗星尾部DNA（TailDNA）含量能否预测苯接触工人外周血细胞数量的变化规律.方法 于2011年,采用整群抽样方法,选取150名某石化厂男性接苯工人,采集研究对象肘静脉血和尿分别进行血常规检测、彗星试验、苯巯基尿酸（SPMA）和尿肌酐浓度检测.分别以尿SPMA及TailDNA对人群进行分组,追踪其后3年的血常规资料,分析研究对象血细胞数量变化趋势.结果 低SPMA组工人不同年份白细胞（WBC）、中性粒细胞（NEUT）计数均明显低于高SPMA组,差异均有统计学意义（P＜0.05）;低TailDNA组2014年血小板（PLT）计数明显高于2012年（P＜0.05）,而高TailDNA组中差异无统计学意义（P＞0.05）;4年间高TailDNA组RBC计数均低于低TailDNA组,差异有统计学意义（P＜0.05）.高TailDNA组WBC计数随时间变化呈下降趋势（β=-0.113,P＜0.05）,低TailDNA组WBC计数变化趋势无统计学意义（P＞0.05）.结论 TailDNA可能用于预测苯接触人群血细胞数量变化规律.Objective To investigate the value of DNA content in comet tail （TailDNA） in predicting the changes in peripheral blood cell counts in workers exposed to benzene.Methods In 2011,cluster sampling was used to select 150 male workers exposed to benzene in a petrochemical factory.Cubital venous blood and urine samples were collected for routine blood rest,comet assay,and measurement of s-phenylmercapturic acid （SPMA） and urine creatinine.The population was divided into groups according to urinary SPMA or TailDNA,and routine blood test results within 3 years were collected to analyze the changes in blood cell counts.Results The low-SPMA group had significantly higher white blood cell and neutrophilcounts in all years than the high-SPMA group （P〈 0.05）.The low-Tail DNA group had a significant increase in platelet count from 2012 to 2014 （P〈0.05）,while the high-Tail DNA group had no significant change （P〉0.05）.During the 4-year period,the high-TailDNA group had a significantly lower red blood cell count than the low-TailDNA group （P〈0.05）.The high-TailDNA group showed a gradual reduction in white blood cell count over time （β=-0.113,P〈0.05）,and the low-TailDNA group showed no trend of the reduction in white blood cell count （P〉0.05）.Conclusion TailDNA can be used to predict the changes in blood cell counts in workers exposed to benzene.

关 键 词：苯 职业暴露 彗星尾部DNA 血细胞计数 

分 类 号：R135[医药卫生—劳动卫生]