维吾尔族人群外周血DNA提取质量因素分析  

Analysis on quality factors of DNA extracted from peripheral blood of Uygur population

作　　者：刘树虎[1] 热比亚木.巴克 布帕提玛穆.阿布都克热穆 多力坤.买买提玉素甫 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《实验技术与管理》2017年第5期64-67,共4页Experimental Technology and Management

基　　金：国家自然科学基金项目"新疆隔离人群的遗传多样性研究及流行病学资源数据库建立"(31460285);新疆维吾尔自治区自然科学基金项目"新疆阿尔克孜族隔离人群减数分裂重组热点遗传定位研究"(2013211A016);中国科学院计算生物学重点实验室开放课题(2014KLCB02)

摘　　要：使用改良盐析法、改良酚/氯仿法、改良碘化钾法和试剂盒法,分别对在-80℃保存1~4年的维吾尔族人群血样提取DNA,分光光度仪检测DNA浓度和纯度,0.8%琼脂糖电泳检测,提取的DNA质量采用单因素方差分析比较。结果表明,改良碘化钾法提取DNA质量最好,其次是试剂盒法、酚/氯仿提取法,盐析法提取质量最差;冻存1~4年的血样提取的DNA浓度之间差异极显著(F=618.011,P=0.000),冻存1~4年的血样提取的DNA纯度之间差异极显著(F=27.090,P=0.000)。该研究基于经济实惠、方便操作,提取DNA质量的高低可以确定改良碘化钾法提取DNA质量最好;血样本冻存(-80℃)超过3年对提取的DNA质量有显著的影响。Objective： It is to analyze the factors affecting the quality of DNA extracted from the genomic DNA of whole blood frozen samples in Uygur population by different samples and freezing time.Methods： The blood samples at-80 ℃ for 1-4 years are extracted by the modified salting-out method, the modified phenol/chloroform method, the KI method and reagent box method.The concentration and purity of DNA are detected by the spectrophotometer and 0.8% agarose gel electrophoresis, and the extracted DNA quality is compared by the single factor variance analysis.Results： The improved KI method is the best to extract DNA, followed by the reagent box method and phenol/chloroform method, and the extraction quality by the salting-out method is the worst.The concentration of DNA extracted from the frozen blood samples for 1-4 years is significantly different （F=618.011, P=0.000） and so is its purity（F=27.090, P=0.000）.Conclusion： The research is based on economic benefit and easy operation.The quality of DNA can be determined by the improved KI method, and the blood samples frozen （-80 ℃） for more than 3 years have significant effect on the quality of extracted DNA.

关 键 词：维吾尔族人群 冻存外周血 DNA提取 DNA质量 单因素方差分析 

分 类 号：Q987[生物学—遗传学]