泡核桃SSR标记开发及在遗传多样性研究中的应用  

Development of SSR Markers in Juglans sigillata and Its Application in Genetic Diversity Analysis

作　　者：陈少瑜[1,2] 宁德鲁[1,2] 吴涛[1,2] 肖良俊[1,2] 林红丁 朱云凤[1,2] 贺娜[1,2] 潘莉[1,2] 

机构地区：[1]云南省林业科学院,云南昆明650201 [2]云南省木本油料工程技术研究中心,云南昆明650201 [3]西南林业大学,云南昆明650224

出　　处：《西北林学院学报》2017年第3期91-96,共6页Journal of Northwest Forestry University

基　　金：国家自然科学基金(31660214);国家科技支撑计划课题(2011BAD46B01)

摘　　要：在泡核桃转录组数据分析的基础上开发SSR标记,从中筛选多态性高、稳定性好的标记,对泡核桃、北方核桃及两者的杂交品种(核桃、美国引进核桃以及泡核桃和核桃的杂交品种)共15个品种(系)进行遗传多样性分析。结果表明,开发的11对引物对15个样本扩增结果多态性较高,稳定性较好,多态性信息量PIC平均为0.302 3;基于遗传相似性系数聚类显示15个样本在相似性系数为0.33处聚为3组。可为进一步遗传图谱构建、基因定位及分子辅助育种等研究提供科学依据。Based on the transcriptomic data of Juglans sigillata, SSR markers were developed, from which the polymorphic and stable markers were screened out. The genetic diversity of 15 varieties were analyzed, including varieties in J. sigillata,J, regia and their hybrid varieties. The results showed that 11 pairs of developed SSR primers used for formal PCR amplification of 15 varieties had relatively high polymorphism （with average PIC 0. 302 3） and better stability. Cluster analysis showed that 15 samples were divided into 3 groups when the genetic similarity coefficient was 0.33. The study would lay a foundation for the further study of genetic map construction,gene location and molecular assistant breeding selection.

关 键 词：泡核桃 北方核桃 杂交品种 SSR标记 遗传多样性 

分 类 号：S718.43[农业科学—林学]