丝胶对STZ致损伤INS-1细胞活性和胰岛素分泌的影响  

EFFECTS OF SERICIN ON CELL VIABILITY AND INSULIN SECRETION OF STZ INJURED INS-1 CELLS

作　　者：王小杰[1] 刘美晓 谢云鹏[2] 孙一婵 陈志宏[1] 

机构地区：[1]承德医学院,河北承德067000 [2]承德医学院附属医院

出　　处：《承德医学院学报》2017年第3期190-192,共3页Journal of Chengde Medical University

基　　金：国家自然科学基金资助项目(81441133);河北省自然科学基金资助项目(H2013406096);河北省教育厅青年基金项目(QN20131089)

摘　　要：目的:探讨丝胶对STZ致损伤的大鼠胰岛素瘤INS-1细胞活性和胰岛素分泌的影响。方法:以链脲佐菌素(STZ)诱导INS-1细胞损伤,细胞分为正常组、STZ组和丝胶组(300μg/L)。普通光学倒置显微镜观察各组细胞的一般状态;MTT法测定细胞活性;ELISA法检测培养上清中胰岛素的含量。结果:与正常组相比,STZ组细胞活性明显下降,培养上清中胰岛素的含量明显降低(P<0.05)。经300μg/L丝胶处理后,INS-1细胞活性、培养上清中胰岛素的含量均明显升高(P<0.05)。结论:丝胶蛋白可提高STZ致损伤大鼠胰岛素瘤INS-1细胞的活性,以及促进胰岛素的分泌。Objective： To investigate the effects ofsericin on cell viability and insulin secretion of streptozotocin （STZ） injured rats＇ insulinoma INS-1 ceils. Methods： The INS-1 cells were randomly divided into normal group, STZ group and sericin group. The cells in STZ group were cultured with STZ（I 0retool/L） for 24h; the cells in sericin group were cultured with STZ（10mmol/L） and sericin （300 μg/L） at the same time for 24h. The general state of INS-1 cells was observed by optical inverted microscope, MMT to detect the cell viability of INS-1 ceils and ELISA to detect the insulin content in ~. Results： Compared with normal group, the ceil viability and insulin content in supernate of INS-1 cells decreased obviously （P〈0.05）; After treating with sericin （300 μg/L）, the cell viability and insulin content in supemate of INS- l cells increased obviously （P〈0.05）. Conclusions： Sericin can promote insulin secretion by enhancing the ceil viability of STZ injured rats＇ insulinoma INS-1 ceils.

关 键 词：丝胶 链脲佐菌素(STZ) INS-1细胞 细胞活性 胰岛素分泌 

分 类 号：R587.1[医药卫生—内分泌]