香蕉一个钾离子通道基因的克隆与生物信息学分析  

Cloning and Expression Analysis of MaAKT2-like Gene from Banana

作　　者：杨日才 姜成东[1] 

机构地区：[1]海南大学园艺园林学院,海南海口570228

出　　处：《热带农业工程》2017年第1期10-16,共7页Tropical Agricultural Engineering

基　　金：海南省自然科学基金(No.311026);海南大学青年基金(No.qnjj1159);海南大学教育教学改革研究项目(No.hdjy1635);海南省星火产业带专项资金项目(No.HNXH201425)

摘　　要：从香蕉中克隆了一个钾离子通道基因MaAKT2的cDNA的ORE序列并初步分析其功能。采用PCR技术克隆该基因,利用生物信息学方法分析基因及推导蛋白的序列,利用RT-PCR技术对该基因的组织器官表达特性进行分析。结果表明,MaAKT2的cDNA ORE序列长为2 463 bp,编码821个氨基酸;MaAKT2蛋白属于植物弱内整流钾离子通道蛋白,该蛋白具6个跨膜区域,拥有CNBD、ANKY及KHA特征结构域;MaAKT2在系统发生上更接近单子叶植物弱内整流钾离子通道蛋白;MaAKT2可在香蕉根、茎、叶、花以及果实中表达,在叶中的表达量高于其它器官。MaAKT2基因的克隆为在分子水平研究香蕉钾离子吸收利用机制奠定基础。The objective of this study is to clone the ORF of cDNA from a gene MaAKT2 from banana and primarily analyze its function, cDNA sequence was obtained by the approach of PCR amplification, the putative amino acid sequence were analyzed by bioinformatics software and the expression patterns in different organs was analyzed by RT- PCR. Sequence analysis indicated that the ORF of MaAKT2 gene is 2463bp in length and encodes an 861 amino acids protein. MaAKT2 protein belongs to weak inward rectifying potassium channel protein characterized with six transmembrane regions, a CNBD domain, a ANKY domain and a KHA domain. The phylogenetic analysis showed that MaAKT2 had a closer relationship with Anthuriurn amnicola, Ananas comosus, Oryza sativa, and Zea mays; MaAKT2 is expressed in all organs such as roots, corns, leaves, flowers and fruits with higher level in leaves than other organs. The clone of ORF of MaAKT2 is a basement for the study of the mechanical of banana potassium absorption and transport in molecular level.

关 键 词：香蕉 钾离子通道 基因克隆 生物信息学 表达分析 

分 类 号：S188[农业科学—农业基础科学]