胶质纤维酸性蛋白在黄羽肉鸡肠道中的分布研究  

Study on the Distribution of Glial Fibrillary Acidic Protein in Intestine of Yellow Feather Broiler

作　　者：张玉宇[1] 赵新林 祁凤华[1] 陆艳[1] 石晓雨 韩小丽[1] 徐春生[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003 [2]新疆兵团科技局,乌鲁木齐830000

出　　处：《中国畜牧兽医》2017年第5期1315-1320,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金资助项目(31560683);石河子大学高层次人才科研启动资金专项(RCZX201105)

摘　　要：本研究旨在探究胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)在黄羽肉鸡肠道中的分布规律。使用免疫组织化学SABC-AP法,观察鸡肠道中GFAP的分布规律。结果显示,GFAP在鸡小肠黏膜上皮、小肠腺细胞腔面、小肠黏膜下神经丛和肌间神经丛及其血管壁周围均呈强阳性表达,在黏膜固有膜上呈阳性表达;GFAP在鸡大肠黏膜上皮、大肠腺中呈阳性表达,在大肠黏膜下神经丛和肌间神经丛均呈强阳性表达。GFAP是肠神经胶质细胞的特异性标记物之一,观察其在鸡肠道的分布特征有助于阐明肠神经胶质细胞在肠道各段的分布规律,为研究鸡肠神经胶质细胞的功能提供形态学依据。The experiment was conducted to explore the distribution of glial fibrillary acidic protein（GFAP）in Yellow feather broiler,and to investigate the morphological characteristics of glial cells of chicken.The distribution of GFAP was studied by immunohistochemistry SABC-AP method.The results showed that GFAP were expressed strong positively in chicken small intestinal mucosa epithelium,intestinal gland cell cavity surface,submucosal plexus and myenteric plexus;The expressions of GFAP were positive in the mucosal lamina propria and myenteric nerve plexus around the blood vessel;In avian escherichia sticky epithelial membrane,colorectal adenocarcinoma,GFAP were expressed positively,and the expressions were strong positive in mucosa epithelium,submucosal plexus and myenteric plexus.GFAP was one of the specific marker of enteric glial cells,and the observation of distribution of GFAP in chicken intestinal tract was help for elucidating the enteric glial cells in the distribution of the intestine and providing the morphological basis for the study of chicken glial cell function.

关 键 词：黄羽肉鸡 肠神经胶质细胞 胶质纤维酸性蛋白 小肠 大肠 免疫组织化学 

分 类 号：S852.162[农业科学—基础兽医学]