难治性癫痫婴幼儿肠道菌群的变化  

Alterations of gut microbiota in infants with refractory epilepsy

作　　者：王文建[1] 周潜 戴文魁 邱创钊 王和平[1] 路新国[1] 林素芳[1] 叶敬花[1] 马卓娅[1] 

机构地区：[1]深圳市儿童医院,广东深圳518038 [2]深圳微健康基因研究院,广东深圳518129

出　　处：《中国微生态学杂志》2017年第5期502-505,共4页Chinese Journal of Microecology

基　　金：深圳市科创委基础研究项目(JCYJ20150403100317071)

摘　　要：目的探讨难治性癫痫婴幼儿的肠道菌群特点。方法研究纳入年龄1~3岁的确诊难治性癫痫婴幼儿14例,同时匹配同年龄段健康婴幼儿30例作为对照。留取受试者的粪便标本,提取总DNA并扩增,对16S rRNA基因进行高通量测序,对测序结果进行生物学分析。结果两组婴幼儿样本门水平的优势菌群主要有厚壁菌、拟杆菌、变形菌和放线菌四类,癫痫组婴幼儿拟杆菌和放线菌所占比例明显低于健康组(28.60%vs 53.19%,2.58%vs 8.50%),而厚壁菌和变形菌的比例则明显高于健康组(43.19%vs 34.00%,24.87%vs 3.09%)。癫痫组婴幼儿Cronobacter属和Erysipelatoclostridium属的比例高于健康组(23.59%vs 0.86%,9.50%vs 0.76%),而拟杆菌和双歧杆菌的比例明显低于健康组(19.44%vs 42.84%,1.04%vs 7.85%)。结论婴幼儿难治性癫痫存在肠道微生态失衡,尚需进一步研究肠道菌群在难治性癫痫发病中的作用。Objective To explore the characteristics of gut microbiota of children with refractory epilepsy vs healthy controls. Methods We collected stool samples from 14 infants aged from 1 year to 3 years with refractory epilepsy （Seizures group） and 30 age matched controls （Health group） . Bacterial DNA was extracted from their fecal samples and the V3 - V4 hypervariable regions of 16S rRNA were sequenced using the Illumina MiSeqTMII system. Results The predominant intestinal microbiota in all the infants included the phyla Firmicutes, gacteroidetes, Proteobacteria and Actinobacteria. Compared to those of the Health group, the proportions of the phyla Bacteroidetes （28.60% vs 53.19%%） and Actinobacteria （2.58% vs 8.5%） in Seizures group were less, while those of Firmicutes （43. 19% vs 34.00%） and Proteobacteria （24.87% vs 3.09%） were greater; the proportions of genera Cronobacter （23.59% vs 0.86%） and Erysi- pelatoclostridium （9.50% vs 0. 76 %） in Seizures group were greater, and those of Bacteroides （19.44% vs 42.84%） and Bifidobacterium （1.04% vs 7.85%） were less. Conclusion These findings suggest that the gut microbiome is altered in refractory epilepsy. Further study is needed to better understand their role in the etiopathogenesis of refractory epilepsy.

关 键 词：肠道菌群 难治性癫痫 婴幼儿 高通量测序 

分 类 号：R378[医药卫生—病原生物学]